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SAB4301135

Sigma-Aldrich

Anti-FLAG Tag antibody produced in rabbit

affinity isolated antibody

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

all

Concentration

1.4 mg/mL

Technique(s)

western blot: 1:1000-1:5000 (Cell Lysate)

Isotype

IgG

Séquence immunogène

DYKDDDDK conjugated to KLH (synthetic peptide)

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

The FLAG epitope is composed of eight amino acids with the following sequence AspTyrLysAspAspAspAspLys. It helps in immunoaffinity purification of fusion proteins.

Spécificité

The antibody detects transfected proteins contanining FLAG tag.

Immunogène

Synthetic peptide: DYKDDDDK conjugated to KLH

Application

Anti-FLAG Tag antibody produced in rabbit has been used in:
  • western blotting
  • co-immunoprecipitation
  • immunofluorescence studies
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Learn more product details in our FLAG® application portal.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Informations légales

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Xuan Yang et al.
Cancer science, 110(7), 2133-2144 (2019-05-09)
Lymphoid-specific helicase (LSH) is overexpressed in tumor tissues and its overexpression is associated with poor prognosis in several cancers. However, the role and molecular mechanism of LSH in hepatocellular carcinoma (HCC) remains largely unknown. Herein, we report that LSH was
Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling
Cao Y, et al.
Biochimica et Biophysica Acta, 1863(12), 3148-3159 (2016)
Fajiu Wang et al.
American journal of translational research, 11(11), 7166-7185 (2019-12-10)
Mechanism by which CCNB1 regulates the cell cycle progression and its prognostic function in non-squamous non-small cell lung cancer (NSCLC) are necessary to be further elucidated. Data retrieved from gene expression omnibus (GEO) and cancer genome atlas (TCGA) combined with
Zhen Wei et al.
Nature communications, 9(1), 4468-4468 (2018-10-28)
The conversion of serine and glycine that is accomplished by serine hydroxymethyltransferase 2 (SHMT2) in mitochondria is significantly upregulated in various cancers to support cancer cell proliferation. In this study, we observed that SHMT2 is acetylated at K95 in colorectal
Jung-Ho Shin et al.
Nature communications, 7, 12612-12612 (2016-08-27)
Bacteria respond dynamically to the changes in zinc availability. Repression by the Bacillus subtilis transcription factor Zur requires Zn(II), which binds with negative cooperativity to two regulatory sites per dimer to form, sequentially, Zur2:Zn3 and Zur2:Zn4 forms of the repressor.

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