While this antibody hasn't been validated for flow cytometry, independent laboratories have published its use for this application. For example:
https://www.nature.com/articles/s42003-020-1072-4.pdf
F1804
Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse
1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
ANTI-FLAG® M2 antibody, Mouse monoclonal
Synonyme(s) :
Anticorps FLAG, Anti-ddddk, Anti-dykddddk
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A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
M2, monoclonal
Application:
—
Citations:
9676
Le tarif et la disponibilité ne sont pas disponibles actuellement.
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Laissez-nous vous aiderbiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
purified by
affinity chromatography (affinity tag purification)
species reactivity
all
concentration
1.0-1.2 mg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
General description
The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purification
Method of purification - Affinity tag purification
Immunogen
FLAG ; séquence peptidique DYKDDDDK
Application
Pour la détection spécifique et hautement sensible des protéines de fusion FLAG par immunoblotting, immunoprécipitation (IP)[1], immunohistochimie, immunofluorescence et immunocytochimie. Optimisé pour la détection en bande simple de protéines de fusion FLAG dans des systèmes d'expression bactériens, végétaux et de mammifères.
Western blotting et EIA
Pour en savoir plus sur nos produits, rendez-vous sur notre portail des applications des produits FLAG®.
Western blotting et EIA
Pour en savoir plus sur nos produits, rendez-vous sur notre portail des applications des produits FLAG®.
Biochem/physiol Actions
Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Physical form
Contains 50% glycerol in phosphate buffered saline.
Preparation Note
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Cet article | |||
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form - | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| conjugate unconjugated | conjugate - | conjugate FITC conjugate | conjugate CY3 conjugate |
| biological source mouse | biological source - | biological source mouse | biological source mouse |
| clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal |
| species reactivity all | species reactivity - | species reactivity all | species reactivity all |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Classe de stockage
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Can this Antibody be used for Flow cytometry?
1 answer-
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Any protocol available for using the Anti-Flag M2 (F1804) in a ChIP-Seq assay? And, if the lysis buffer contains SDS and sodium deoxycholate at a concentration of 0.1%, will the presence of both denature or interfere with binding in the research process?
1 answer-
Several papers have cited F1804 in ChIP & ChIPseq applications:
https://www.nature.com/articles/s41467-023-40803-y.pdf
https://www.nature.com/articles/s41467-023-41307-5.pdf
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10541012&blobtype=pdf
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10541012&blobtype=pdf
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10693086&blobtype=pdf
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10863984&blobtype=pdfAnti-FLAG® M2 Magnetic Beads are not compatible with deoxycholate and SDS. Please refer to compatibility chart on the datasheet linked below for further information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/075/m8823bul-ms.pdfHelpful?
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Is this Ab suitable for ELISA?
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This antibody is compatible with enzyme immunoassay, which includes ELISA, the most widely used form of EIA.
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Bonjour, il est indiqué que cet anticorps marche en immunohistochimie. Quel protocole d'antigen retrieval faut-il utiliser et quelle concentration d'anticorps sur coupe FFPE? Merci
1 answer-
Please see the reference at the link below using this antibody at 1:50 dilution in IHC with antigen retrieval in citrate buffer for 5 minutes.
https://www.nature.com/articles/s41467-019-12187-5.pdfHelpful?
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モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。
1 answer-
An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdfHelpful?
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