F2555 will recognize N-terminal and C-terminal FLAG fusion proteins.
F2555
Rabbit Monoclonal ANTI-FLAG® Clone SIGI-25
clone SIG1-25, ascites fluid
Synonyme(s) :
Anti-ddddk, Anti-dykddddk
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A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SIG1-25, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
133
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Laissez-nous vous aiderbiological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SIG1-25, monoclonal
technique(s)
immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100, indirect ELISA: suitable, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein
isotype
IgG
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG® peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG® peptide is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme. This rabbit ANTI-FLAG® antibody is produced from the hybridoma SIG1-25. The hybridoma is derived by the fusion of myeloma cells and splenocytes from rabbits immunized with the FLAG® peptide sequence conjugated to KLH.
Immunogen
FLAG peptide sequence DYKDDDDK
Application
Monoclonal ANTI-FLAG® antibody produced in rabbit has been used in:
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Biochem/physiol Actions
This monoclonal antibody recognizes the FLAG sequence at the N-terminus
Physical form
Provided as ascites fluid containing 15 mM sodium azide as a preservative.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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| description clone SIG1-25, ascites fluid | description clone M1, purified immunoglobulin, buffered aqueous solution | description clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide) | description clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate) |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate CY3 conjugate |
| clone SIG1-25, monoclonal | clone M1, monoclonal | clone M2, monoclonal | clone M2, monoclonal |
| biological source rabbit | biological source (Bioreactor) | biological source mouse | biological source mouse |
| antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified immunoglobulin (Purified IgG1 subclass) | antibody form purified immunoglobulin |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| technique(s) immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein, indirect ELISA: suitable | technique(s) western blot: 10 μg/mL | technique(s) western blot: 10 μg/mL (Protein A) | technique(s) direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone |
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Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Will Product No. F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit - clone SIG1-25, recognize an internal FLAG tag protein?
1 answer-
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Is Product F2555 a monoclonal or polyclonal FLAG® antibody?
1 answer-
F2555 is a monoclonal antibody, raised in rabbits. [Note: Nearly all of the monoclonal antibodies sold by Sigma-Aldrich are raised in mice.]
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What positive controls can be used for immunoblotting with Product F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit - clone SIG1-25?
1 answer-
Two of the FLAG-tagged proteins offered by Sigma-Aldrich are the Amino-terminal FLAG-BAP fusion protein (Product No. P7582) and the Carboxy-terminal FLAG-BAP fusion protein (Product No. P7457). Both of these proteins should work with the F2555 rabbit ANTI-FLAG.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why should I use Product No. F2555, Monoclonal ANTI-FLAG® antibody produced in rabbit?
1 answer-
The main application for Product F2555 rabbit monoclonal is for immunoblotting after immunoprecipitation with M2, which is a mouse antibody. This enables using a secondary antibody (anti-rabbit) that will not bind the M2 mouse heavy (50 kD) and light chains (25 kD).
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