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F4042

Sigma-Aldrich

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

Numéro MDL:
MFCD00282246
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.32

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

M5, monoclonal

Forme

buffered aqueous solution

Produit purifié par

using Protein A

Espèces réactives

all

Concentration

2-5 mg/mL

Technique(s)

western blot (chemiluminescent): 10 μg/mL

Isotype

IgG1

Séquence immunogène

DYKDDDDK

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

Immunogène

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Forme physique

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Notes préparatoires

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Autres remarques

Antibody is not calcium dependent.

Informations légales

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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The Journal of Biological Chemistry, 289(5), 2852-2863 (2014)
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