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P4860

Sigma-Aldrich

Protéase from Bacillus licheniformis

≥2.4 U/g

Synonyme(s) :

Protéinase from Bacillus licheniformis, Subtilisine A

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About This Item

Numéro CAS:
9014-01-1
Numéro de classification (Commission des enzymes):
3.4.21.62 (BRENDA, IUBMB)
Numéro CE :
232-752-2
Numéro MDL:
MFCD00132092
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Forme

aqueous solution

Niveau de qualité

200

Activité spécifique

≥2.4 U/g

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.[1]

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations.
The enzyme from Sigma has been used to prepare chicken (leg) bone protein hydrolysates while evaluating ACE inhibitory peptides.[2] It has also been used for the hydrolysis of Chlorella pyrenoidosa protein extracts during a study of their antitumor activities.[3]

Actions biochimiques/physiologiques

Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Remarque sur l'analyse

Activity expressed in Anson Units

Informations légales

A product of Novozyme Corp.
Alcalase is a registered trademark of Novozymes Corp.

Mention d'avertissement

Danger

Mentions de danger

H315,H318,H334,H335,H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
0000409449
0000409449
0000404725
0000383620
0000378840

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Consulter la Bibliothèque de documents

Andrew R Hirst et al.
Nature chemistry, 2(12), 1089-1094 (2010-11-26)
Supramolecular gels, which demonstrate tunable functionalities, have attracted much interest in a range of areas, including healthcare, environmental protection and energy-related technologies. Preparing these materials in a reliable manner is challenging, with an increased level of kinetic defects observed at
Srinidhi Jayaraman et al.
The Journal of pharmacology and experimental therapeutics, 379(2), 191-202 (2021-08-15)
Neurolysin (Nln) is a recently recognized endogenous mechanism functioning to preserve the brain from ischemic injury. To further understand the pathophysiological function of this peptidase in stroke and other neurologic disorders, the present study was designed to identify small molecule
Keiichi Yokoyama et al.
Applied microbiology and biotechnology, 87(6), 2087-2096 (2010-06-04)
Microbial transglutaminase (MTG) has been used extensively in academic research and the food industries through its cross-linking or posttranslational modification of proteins. Two enzyme engineering approaches were applied to improve MTG activity. One is a novel method of rational mutagenesis
Catherine Cesa-Luna et al.
PloS one, 15(10), e0240545-e0240545 (2020-10-16)
The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production
Proteases: multifunctional enzymes in life and disease
Lopez-Otin C and Bond JS
Test, 283(45), 30433-30437 (2008)
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Questions

1–10 of 13 Questions  

  1. Is this a food grade product?

    1 answer

    1. This product is intended for research use only and is not suitable for human or veterinary use. Currently, a food grade product is not available.

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  2. What is the density of the P4860 enzyme?

    1 answer

    1. This item is a product of Novozymes Corp. The supplier reports a density of 1.17g/ml at 20 degrees C.

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  3. What is optimal temperature and ph for this enzyme?

    1 answer

    1. According to the manufacturer’s (Novozymes Corp.) Product Sheet, by analyzing Alcalase at various pH values using a modified Anson/haemoglobin method, its optimum activity has been found to be at pH between 6.5 and 8.5; its temperature optimum is around 60°C, but could vary between 55°C and 70°C, depending on the type of substrate.

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  4. How many mg of enzyme/mL of solution are there?

    1 answer

    1. The density of the solution is 1.17 g/mL at 20 °C.

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  5. Can this be used for production of fish protein hudrolysate? Also what is the difference between this enzymeand Alcalse 2.4l?

    1 answer

    1. This product is identical to Alcalase 2.4L. This material has not been tested for use in fish. A search of the literature shows a number of publications related to the use of this enzyme in animal protein production. However, a specific reference for use in fish was not found. Please see the links below to review articles that may be of interest:

      Chicken meat
      https://ift.onlinelibrary.wiley.com/doi/10.1111/j.1750-3841.2008.00765.x

      Fish scales
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063752/

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  6. What is the extinction coefficient?

    1 answer

    1. The extinction coefficient is not determined for this product.

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  7. What is the extinction coefficient for this enzyme?

    1 answer

    1. The extinction coefficient for this enzyme is not determined.

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  8. What is the molecular weight?

    1 answer

    1. The molecular weight of Protease from Bacillus licheniformis (Product No. P4860) is approximately 27 kDa.

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  9. What is the optimal pH for the use of enzyme P4860?

    1 answer

    1. According to the manufacturer Novozyme, the optimal pH for the use of product P4860 is approximately pH 9.

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  10. What is the concentration of the product, and how can the conversion from units/g on the COA to units/ml be performed?

    1 answer

    1. The density of the Novozymes product, as indicated in the SDS sheet, is 1.17g/ml. By multiplying 1.17 g/ml (density) by 50 ml (pack size), 58.5 g of the solution is obtained. Further multiplication with 2.55 unit/g (lot activity) results in 149.175 or 149.2 units. Dividing 149.2 units by 50ml gives 2.984 units/ml.

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1–10 of 13 Questions  

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