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M6770

Sigma-Aldrich

MCDB 201 Medium

With trace elements, L-glutamine and 30 mM HEPES, powder, suitable for cell culture

Synonyme(s) :

MCDB medium

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About This Item

Code UNSPSC :
41161501
Nomenclature NACRES :
NA.75

Niveau de qualité

500

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Composants

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

MCDB 201 medium is a modified Ham′s nutrient mixture F-12 that is synthesized for the clonal growth of chicken embryo fibroblasts.

Application

MCDB 201 Medium has been used as a component of:
  • the hepatic differentiation medium to culture pluripotent stem cells,
  • epididymal growth medium for culturing of preadipocytes,
  • Dulbecco′s Modified Eagle Medium (DMEM)/F12 for culturing of human umbilical cord mesenchymal stem cells

Quantité

Formulated to contain 17.7 grams of powder per liter of medium.

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Jeff Ishibashi et al.
Molecular and cellular biology, 32(12), 2289-2299 (2012-04-05)
Fibroblastic preadipocyte cells are recruited to differentiate into new adipocytes during the formation and hyperplastic growth of white adipose tissue. Peroxisome proliferator-activated receptor γ (PPARγ), the master regulator of adipogenesis, is expressed at low levels in preadipocytes, and its levels
Baris Ulum et al.
Journal of cellular physiology, 233(11), 8429-8436 (2018-05-26)
Bone marrow mesenchymal stem cells (BM-MSCs) are promising candidates for regenerative medicine purposes. The effect of obesity on the function of BM-MSCs is currently unknown. Here, we assessed how obesity affects the function of BM-MSCs and the role of endoplasmic
Philip Roelandt et al.
Methods in molecular biology (Clifton, N.J.), 997, 141-147 (2013-04-03)
Differentiation of human stem cells to hepatocytes is crucial for industrial applications as well as to develop new therapeutic strategies for liver disease. The protocol described here, using sequentially growth factors known to play a role in liver embryonic development
Jeff Ishibashi et al.
Molecular and cellular biology, 32(12), 2289-2299 (2012-04-05)
Fibroblastic preadipocyte cells are recruited to differentiate into new adipocytes during the formation and hyperplastic growth of white adipose tissue. Peroxisome proliferator-activated receptor γ (PPARγ), the master regulator of adipogenesis, is expressed at low levels in preadipocytes, and its levels
Philip Roelandt et al.
PloS one, 5(8), e12101-e12101 (2010-08-17)
Stem cell-derived hepatocytes may be an alternative cell source to treat liver diseases or to be used for pharmacological purposes. We developed a protocol that mimics mammalian liver development, to differentiate cells with pluripotent characteristics to hepatocyte-like cells. The protocol
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