Accéder au contenu
Merck

Passer à

A8960

Acide L-ascorbique 2-phosphate sesquimagnesium salt hydrate

≥95%

Synonyme(s) :

Ascorbylphosphate de magnésium, MAP

pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Changer de vue
À vous/RéférenceDisponibilitéPrix

A propos de cet article

Formule empirique (notation de Hill) :
C6H6Mg1.5O9P · xH2O
Numéro CAS:
Poids moléculaire :
289.54 (anhydrous basis)
MDL number:
UNSPSC Code:
12352205
PubChem Substance ID:
NACRES:
NA.79
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider


biological source

synthetic (organic)

Quality Segment

assay

≥95%

form

powder

technique(s)

HPLC: suitable

color

white to off-white

solubility

water: 50 mg/mL

storage temp.

room temp

SMILES string

O=C1O[C@@]([C@@H](O)CO)([H])C([O-])=C1OP([O-])([O-])=O.O=C2O[C@@]([C@@H](O)CO)([H])C([O-])=C2OP([O-])([O-])=O.O.[Mg+2].[Mg+2].[Mg+2]

InChI

1S/2C6H9O9P.3Mg.H2O/c2*7-1-2(8)4-3(9)5(6(10)14-4)15-16(11,12)13;;;;/h2*2,4,7-9H,1H2,(H2,11,12,13);;;;1H2/q;;3*+2;/p-6/t2*2-,4+;;;;/m00..../s1

InChI key

HKRNHMZODRSAPN-IXNKEUJHSA-H

General description

Le sel de sesquimagnésium de l'acide L-ascorbique 2-phosphate hydraté, aussi appelé ascorbylphosphate de magnésium (MAP), est un dérivé stable de la vitamine C (acide L-ascorbique). Le MAP est employé dans différents domaines de recherche, notamment les expériences à base d'émulsions, la déphosphorylation biocatalytique, la différenciation cellulaire et l'ingénierie tissulaire. Son usage particulièrement vaste s'étend jusqu'à la répression génique, les études de transcription et les recherches sur les facteurs de croissance. Les applications potentielles du MAP en biologie cellulaire sont l'induction de modifications morphologiques chez des cellules en culture et l'influence des voies de la mort cellulaire.

Le MAP a en outre un rôle déterminant dans la recherche en métabolomique et en biochimie. En tant qu'antioxydant, il piège efficacement les radicaux libres et protège ainsi contre le dommage oxydatif. Ses propriétés anti-inflammatoires contribuent à réduire l'inflammation et des études ont mis en évidence sa capacité à stimuler la production de collagène. Le MAP sert aussi d'agent chélatant, ce qui élargit encore son domaine d'application à diverses études de recherche. Les multiples facettes du MAP en font un outil précieux pour les investigations scientifiques et contribuent à faire progresser la recherche en biologie cellulaire, en métabolomique et en biochimie.

Application

Le sel de sesquimagnésium hydraté de l′acide L-ascorbique 2-phosphate a été utilisé dans un milieu d′expansion de cellules souches mésenchymateuses (CSM) pour incuber des structures [1], et dans du milieu de Eagle modifié par Dulbecco (DMEM) pour la culture de cellules de cordon ombilical humain  et de cellules souches mésenchymateuses.

Biochem/physiol Actions

L′acide L-ascorbique 2-phosphate (AA2P) est utilisé dans la déphosphorylation biocatalytique destinée à la production d′électricité et dans les tests de détection électrochimiques. L′acide L-ascorbique 2-phosphate peut servir dans les applications de différenciation cellulaire et de modification de tissus. L′Asc-2P est employé dans les études de répression de gènes, par exemple la répression de l′expression de dickkopf-1 induite par la dihydrotestostérone.

Features and Benefits

  • Produit pouvant être utilisé dans la recherche en biologie cellulaire, en métabolomique et en biochimie.
  • Composé de grande qualité convenant à de multiples applications de recherche.

Other Notes

Pour de plus amples informations sur notre gamme de Produits biochimiques, merci de compléter ce formulaire.

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 1

Cet article
A19684975294417
technique(s)

HPLC: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

assay

≥95%

assay

95.0-100.5%

assay

≥95.0% (HPLC)

assay

≥90% (T)

biological source

synthetic (organic)

biological source

synthetic

biological source

synthetic

biological source

-

form

powder

form

powder

form

powder

form

powder

solubility

water: 50 mg/mL

solubility

-

solubility

-

solubility

-

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-


Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

Si vous avez besoin d'assistance, veuillez contacter Service Clients

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents



Questions

1–10 of 13 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How long is stable at -20 or -80 degrees, if dissolved in water or media? By any chance, did you tested the stability of this product at -20 °C or -80 °C?

    1 answer
    1. Unfortunately, the solution stability of this product at freezer temperatures has not been determined. This product is soluble in water at 50 mg/ml. Stock solutions have been found to retain 85% activity for 1 week at 37 °C and 80% activity for 1 month at refrigerated temperatures.

      Helpful?

  4. To whom it may concern, I would like to know more on the biocompatibility of L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate. Could you please send me an example of certificate of analysis for this compound? Thanks a lot. S. Chatellier

    1 answer
    1. Please see the link below to access a specific lot or review a sample Certificate:
      https://www.sigmaaldrich.com/product/sigma/A8960#product-documentation

      Helpful?

  5. What type of filter membrane can be used to sterilize ascorbic acid?

    1 answer
    1. After reconstitution in water, it is recommended to sterilize the solution using a 0.22 micron PES filter, such as one of the following products, or any other dimension that better fits the end user's needs.
      https://www.sigmaaldrich.com/substance/steritopthreadedbottletopfilter1234598765

      Helpful?

  6. Is A8960 sterile? or can i find a sterile alternative?

    1 answer
    1. This is not a sterile product. Stock solutions should be filter-sterilized. The compound is soluble in water at 50 mg/mL. The solution stability has not been determined.

      Helpful?

  7. What is the shelf life/stability since purchased? In powder condition in its original container, at room temperature

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  8. Can stock solutions of this compound be frozen and retain their activity for extended periods of time?

    1 answer
    1. The product stability has not been determined in freezer temperature. Stock solutions that are stored at 5 °C are approximately 80% active after 4 weeks.

      Helpful?

  9. How long is A8960 stable in water when frozen at -20C?

    1 answer
    1. This product stability has not been determined in freezer temperature. Stocks solutions that are stored at 5 °C are approximately 80% active after 4 weeks.

      Helpful?

  10. How long is stable at -20 or -80 degrees, if dissolved in water or media ? Thinking to prepare a stock solution. Thank you

    1 answer
    1. This product is soluble in water at 50 mg/ml, forming a clear colorless to yellow solution. Stocks solutions that are stored at 5 °C are approximately 80% active after 4 weeks.

      If this product is reconstituted or diluted into media, it retains approximately 85% of its activity after 1 week at 37 °C and approximately 80% for 1 month at 5 °C.

      This product stability has not been determined at -20 °C or -80 °C.

      Helpful?

1–10 of 13 Questions  

Reviews

No rating value

Active Filters