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Principaux documents

GERPN6302

Iodoacetamide

Cytiva RPN6302, pack of 25 g

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Forme

solid

Caractéristiques

wetted part: no

Conditionnement

pack of 25 g

Fabricant/nom de marque

Cytiva RPN6302

Couleur

Yellowish

Solubilité

cold water: soluble (Easily soluble in cold and hot water)

Température de stockage

2-8°C

Catégories apparentées

Description générale

Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.

Application

Iodoacetamide has been used:
  • as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
  • in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
  • as a protein alkylation agent

Actions biochimiques/physiologiques

Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Caractéristiques et avantages

  • Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H301,H334,H317,H413

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

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Certificats d'analyse (COA)

Lot/Batch Number

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Les clients ont également consulté

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Iodoacetic acid ≥98.0% (T)

Sigma-Aldrich

I4386

Iodoacetic acid

Differential effects of iodoacetamide and iodoacetate on glycolysis and glutathione metabolism of cultured astrocytes
Schmidt M and Dringen R
Frontiers in Neuroenergetics, 1(13), 1-1 (2009)
Proteomic analysis of palmitoylated platelet proteins
Dowal L, et al.
Blood, 118(13), e62-e73 (2011)
COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal
Xu P, et al.
eLife, 6, e28342-e28342 (2017)
Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction
Roura-Martinez D, et al.
Journal of drug and alcohol research, 6(3), b1-b8 (2014)

Protocoles

Rehydration Solutions

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

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