E7885

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

• Synonyme(s) : Elastase from hog pancreas, Pancreatopeptidase E
• Numéro CAS: 39445-21-1
• Numéro de classification (Commission des enzymes): 3.4.21.36 (BRENDA, IUBMB)
• Numéro CE : 254-453-6
• Numéro MDL: MFCD00130998
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: Porcine
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥4 units/mg protein
• Poids mol.: 25.9 kDa
• Conditionnement: pkg of 1 μmol
• Technique(s): cell culture | mammalian: suitable
• pH: 8
• Adéquation: suitable for
• Activité étrangère: Trypsin ≤50 BAEE units/mg protein
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Application

Elastase from porcine pancreas is used for the following applications:

• Cell culture techniques
• ELISA techniques
• Used in assay for elastase inhibitory activity

Actions biochimiques/physiologiques

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Conditionnement

Package size based on protein content

Définition de l'unité

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable