93595

Bleu de trypan solution

0.4%, for microscopy

• Formule empirique (notation de Hill): C₃₄H₂₄N₆Na₄O₁₄S₄
• Numéro CAS: 72-57-1
• Poids moléculaire : 960.81
• Beilstein: 4360496
• Numéro MDL: MFCD00003969
• Code UNSPSC : 12171500
• ID de substance PubChem : 329770254
• Nomenclature NACRES : MA.02

Propriétés

• Qualité: for microscopy
• Niveau de qualité: 200
• Forme: liquid
• Durée de conservation: limited shelf life, expiry date on the label
• Concentration: 0.4%
• Technique(s): microbe id | staining: suitable
• Couleur: blue to very dark blue
• Densité: 1.007 g/mL at 20 °C
• εmax: 1.4 at 603 nm in methanol
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• Clé InChI: GLNADSQYFUSGOU-UHFFFAOYSA-J

Description

Description générale

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Application

Trypan blue applications include:

• it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
• employed as a collagen stain in a Van Gieson procedure
• To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
• used as a fluorescent tracer of cell populations in embryology
• used as a tumor promoter modulating permeability of lysosomal membranes

Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Actions biochimiques/physiologiques

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Forme physique

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H350
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Carc. 1B
• Code de la classe de stockage: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type ABEK (EN14387) respirator filter