This product ships at ambient temperature and is stable for relatively short periods at room temperature. There is a 1 - 2 week allowance for transit time. A storage temperature excursion of 2 days is not expected to impact the quality or performance of this material.
10771
Histopaque®-1077
Histopaque®-1077
sterile-filtered, density: 1.077
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Stérilité
sterile-filtered
Niveau de qualité
Forme
liquid
Durée de conservation
Expiry date on the label.
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Densité
1.077
Application(s)
hematology
histology
Température de stockage
2-8°C
Description générale
Le produit Histopaque-1077 est un milieu stérile prêt à l'emploi dépourvu d'endotoxines (tests à l'appui). Sa composition, de densité ajustée à 1,077 g/ml, inclut du polysaccharose et du diatrizoate de sodium. Ce milieu prêt à l'emploi permet de récupérer rapidement les lymphocytes viables et d'autres cellules mononucléées à partir de petits volumes de sang total ou de moelle osseuse.
Application
L'Histopaque-1077 a été utilisé dans les situations suivantes :
- typage d'antigènes de lymphocytes humains (HLA).
- première étape d'isolement avant le dénombrement de lymphocytes T, B et "nuls".
- préparation de suspensions de lymphocytes pures pour la culture cellulaire et des tests de cytotoxicité.
- isolement et purification de cellules mononucléées de sang périphérique (PBMC) et de cellules mononucléées de moelle osseuse (BMMC)[1].
- isolement de lymphocytes humains[2].
- isolement de cellules tumorales circulantes de cancer du sein (CTC) à partir de sang total[3].
- La solution Histopaque-1077 est utilisée avec Histopaque-1119 pour récupérer rapidement les cellules mononucléaires et les granulocytes viables présents dans de petits volumes de sang total.
Caractéristiques et avantages
Histopaque®-1077 présente de nombreux avantages, notamment :
- Recouvrement optimal des cellules viables
- Permet de séparer sélectivement les différents types de cellules sanguines
- Évite la déformation des cellules
- Préserve la viabilité cellulaire
- Interférences minimales avec les cellules exogènes
- Performances reproductibles d'un lot à l'autre
- Produit stérilisé par filtration et trouvé négatif pour la recherche d'endotoxines
- Stable entre 2 et 8 °C à l'abri de la lumière pendant au moins 2 ans.
Principe
Étaler du sang traité avec un anticoagulant sur Histopaque-1077. Durant la centrifugation, agréger les érythrocytes avec du polysaccharose ; ils sédimentent alors rapidement. Les granulocytes deviennent légèrement hypertoniques, ce qui accroît leur vitesse de sédimentation et entraîne la formation d'un culot au fond du tube à centrifuger. Les lymphocytes et les autres cellules mononucléées restent à l'interface plasma/Histopaque-1077.
Informations légales
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
En option
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Erin E Thacker et al.
Vaccine, 27(50), 7116-7124 (2009-09-30)
Targeting viral vectors encoding tumor-associated antigens to dendritic cells (DCs) in vivo is likely to enhance the effectiveness of immunotherapeutic cancer vaccines. We have previously shown that genetic modification of adenovirus (Ad) 5 to incorporate CD40 ligand (CD40L) rather than
Evaluation of respiration with clark type electrode in isolated mitochondria and permeabilized animal cells.
Silva AM and Oliveira PJ
Methods in Molecular Biology, 810, 7-24 (2012)
Ana C Leal et al.
Scientific reports, 7(1), 6438-6438 (2017-07-27)
Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release of tumor-derived extracellular vesicles and the activation of host vascular cells. It was proposed that neutrophil extracellular
Romain A Colas et al.
Journal of immunology (Baltimore, Md. : 1950), 197(11), 4444-4452 (2016-11-02)
Maresin 1 (MaR1) is an immunoresolvent that governs resolution of acute inflammation, and its local metabolism in the context of infectious inflammation is of interest. In this study, we investigated the MaR1 metabolome in infectious exudates and its bioactions in
Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
Boyle JJ et al
The American Journal of Pathology, 174(3), 1097-1108 (2009)
Articles
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Protocoles
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
-
Hello, we had a problem with the refrigerator leading the temperature increasing to 44 degrees Celsius for over the 2 days of weekend, so can our Histopaque-1077 stored in this refrigerator can be used anymore?
1 answer-
Helpful?
-
-
Can Histopaque 10771 be placed in a 37°C water bath to bring it to room temperature after being taken out of the refrigerator?
1 answer-
Histopaque 10771 is normally stored at 2-8°C for long-term storage. However, it can be shipped at ambient temperature, suggesting that it is not easily affected by short-term storage conditions. Therefore, warming it up to 37°C is unlikely to have any detrimental effects on the product.
Helpful?
-
-
Can histopaque-1077 be used to isolate live tumor cells in suspension from dead cells/cell debris?
1 answer-
Histopaque 10771 is primarily intended for isolating mononuclear cells from human blood and bone marrow. It is also utilized in cell culture to eliminate dying cells from hybridomas. However, the effectiveness of Histopaque 10771 or Histopaque 10831 in purifying live cells from deceased liver cells remains untested. Percoll and OptiPrep are common alternative methods.
Helpful?
-
-
Does Histopaque®-1077 work for mouse blood, or is Ficoll®-Paque Premium 1.084 a better choice?
1 answer-
While Histopaque 10771 can be used, it is important to note that quality control testing for Histopaque 10771 does not include testing with mouse blood. The preferred products for isolating mononuclear cells from rat and mouse blood are Ficoll Paque Premium 1084 (GE17-5446-02) or Histopaque 10831. These products are specifically formulated for rats, mice, and other small mammals for the higher percentage of cell recovery.
Helpful?
-
-
What impact does freezing Histopaque or Ficoll solutions have on PBMC purification? Will the freeze/thaw cycle affect product performance in assays, or will it continue to function as expected?
1 answer-
It is recommended to avoid freezing Histopaque or Ficoll Paque solutions. Thawing can be achieved by leaving the bottles at room temperature, followed by gentle inversion to mix the solution after thawing.If the solution is clear, particle-free, and the bottle is intact, the product is suitable for use. However, if the bottle is cracked or broken, it should be replaced.
Helpful?
-
-
If Histopaque-1077 is brought to room temperature, how long can it be left at that temperature before use? Specifically, will leaving it for a few hours cause any issues?
1 answer-
Ficoll Paque has a storage temperature range of 4°C to 30°C for unopened bottles, while opened bottles should be stored at 4°C to 8°C. However, Histopaque can be stored at room temperature for short periods.
Helpful?
-
-
What is the percentage of peripheral blood mononuclear cells (PBMCs) recovered from human blood?
1 answer-
Generally, Histopaque recoveries typically range from 50% to 80%. The percentage of recovery varies significantly depending on the total white cell count of the patient. Higher white cell counts typically have the best recovery rates. Yields are also generally higher with fresh blood and lower with older blood.
Helpful?
-
-
Can Histopaque 10771 be utilized to separate tumor cells from monocytes?
1 answer-
Histopaque 10771 is unlikely to be suitable for separating tumor cells from monocytes. It is designed to separate monocytes and lymphocytes from human blood. Unless the density of your specific tumor cells significantly differs from 1.077 g/ml, there is a possibility that tumor cells may be collected within the mononuclear cell band. Alternatives such as Percoll, Percoll Plus, or OptiPrep could be better options.
Helpful?
-
-
Are Histopaque 1077 and Ficoll Paque pure products? What is the percentage of polysaccharide or polysucrose in Histopaque 1077 and Ficoll Paque?
1 answer-
Both Histopaque and Ficoll Paque are formulated with a combination of polysucrose 400 and sodium diatrizoate. On average, Histopaque and Ficoll Paque solutions contain approximately 5.7% polysucrose and 9.0% sodium diatrizoate. The final density of these solutions is expected to be around 1.077 g/ml for both products with a typical density variation of +/- 0.001%.
Helpful?
-
-
Can we use a ratio of blood to separation media of 4:3 with Histopaque-1077? Also, can Histopaque-1077 be used to dilute the blood up to 1:2 before layering with separation media with HBSS buffer, and then wash the cells too.
1 answer-
Histopaque 10771/H8889 and Ficoll Paque solutions are produced with similar reagents. As Histopaque has a slightly lower density range, yields might be slightly lower. Therefore, it is a mixed bag and yields with Histopaque might be slightly lower. When observing a very high white cell count, dilution is recommended. The standard dilution for peripheral blood is generally 1:2. For bone marrow, a 1:2 or 1:4 dilution are more commonly used. The expectation is that cell recovery should be almost the same.
Helpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
