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E2129

Sigma-Aldrich

Evans Blue

Dye content ≥75 %, Powder

Synonyme(s) :

Direct Blue 53

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About This Item

Formule empirique (notation de Hill):
C34H24N6Na4O14S4
Numéro CAS:
314-13-6
Poids moléculaire :
960.81
Numéro C.I. (Colour Index):
23860
Numéro CE :
206-242-5
Numéro MDL:
MFCD00004021
Code UNSPSC :
12171500
ID de substance PubChem :
24894456
Nomenclature NACRES :
NA.47

product name

Evans Blue, Dye content ≥75 %

Forme

powder

Composition

Dye content, ≥75%

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;

Clé InChI

ATNOAWAQFYGAOY-GPTZEZBUSA-J

Informations sur le gène

human ... PTPN1(5770) , PTPRF(5792)

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Catégories apparentées

Description générale

Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.

Application

Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.

Actions biochimiques/physiologiques

Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H350

Classification des risques

Carc. 1B

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Peterson RL, et al.
Teaching Plant Anatomy Through Creative Laboratory Exercises (2008)
CO2 is the main inorganic C species entering photosynthetically active leaf protoplasts of the freshwater macrophyte Ranunculus penicillatus ssp. pseudofluitans.
Plant, Cell and Environment, 22, 1019-1026 (1999)
Pilhan Kim et al.
Nature methods, 7(4), 303-305 (2010-03-17)
In vivo imaging of small animals offers several possibilities for studying normal and disease biology, but visualizing organs with single-cell resolution is challenging. We describe rotational side-view confocal endomicroscopy, which enables cellular imaging of gastrointestinal and respiratory tracts in mice
Yulian Zhou et al.
The Journal of clinical investigation, 124(9), 3825-3846 (2014-08-02)
Canonical WNT signaling is required for proper vascularization of the CNS during embryonic development. Here, we used mice with targeted mutations in genes encoding canonical WNT pathway members to evaluate the exact contribution of these components in CNS vascular development
Theodora Agalioti et al.
Nature communications, 8, 15205-15205 (2017-05-17)
Malignant pleural effusion (MPE) is the lethal consequence of various human cancers metastatic to the pleural cavity. However, the mechanisms responsible for the development of MPE are still obscure. Here we show that mutant KRAS is important for MPE induction
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