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Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 5 cm × 2.1 mm, HPLC Column

Synonym(e):

Core-shell-(SPP-)Fused-Core-C18-HPLC-Säule

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About This Item

UNSPSC-Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × ID:
5 cm × 2.1 mm
Oberflächenbereich:
135 m2/g
Partikelgröße:
2.7 μm
Aktive Matrixgruppe:
C18 (octadecyl) phase
Porengröße:
90 Å
Matrix:
Fused-Core particle platform
superficially porous particle
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Produktbezeichnung

Ascentis® Express C18, 2,7 μm HPLC-Säule, 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

Materialien

stainless steel column

Qualitätsniveau

100

Agentur

suitable for USP L1

Produktlinie

Ascentis®

Leistungsmerkmale

endcapped

Hersteller/Markenname

Ascentis®

Verpackung

1 ea of

Parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

Methode(n)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × ID

5 cm × 2.1 mm

Oberflächenbereich

135 m2/g

Verunreinigungen

<5 ppm metals

Matrix

Fused-Core particle platform
superficially porous particle

Aktive Matrixgruppe

C18 (octadecyl) phase

Partikelgröße

2.7 μm

Porengröße

90 Å

Betriebs-pH-Wert

2-9

Anwendung(en)

food and beverages

Trenntechnik

reversed phase

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Verwandte Kategorien

Allgemeine Beschreibung

Ascentis Express HPLC-Säulen bieten durch Fused-Core® Partikeltechnologie die hohe Geschwindigkeit und Effizienz von Partikeln mit weniger als 2 μm Durchmesser, wobei gleichzeitig ein geringer Rückdruck beibehalten wird. Anwender der UPLC® (oder anderer Ultrahochdrucksysteme) und der konventionellen HPLC profitieren von dieser Kombination aus hoher Effizienz und geringem Rückdruck.
Bitte besuchen Sie die Ascentis Express Homepage, um weitere Informationen über diese neue Säulentechnologie zu erhalten.

Anwendung

HPLC-Analyse von Capsaicin in Chilipulver mit Ascentis® Express C18, 2,7 μm
UHPLC/MS-Analyse von Maiskeimöl mit Ascentis® Express C30 im Vergleich zu Ascentis® Express C18
UHPLC-Analyse von Paracetamol und Verunreinigungen mit Ascentis® Express C18, 2,7 μm
HPLC-Analyse von Parabenen mit Ascentis®Express C18, 2,7 μm

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

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Anwendung

Produkt-Nr.
Beschreibung
Preisangaben

G004243

LC/MS Analysis of Plasma Samples on Ascentis® Express C18 Showing Baseline Improvements after Phospholipid Removal using HybridSPE®-Phospholipid, application for SPE, application for LC-MS

G004366

HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid, application for SPE, application for LC-MS

G004432

HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB, application for SPE, application for LC-MS

G005619

LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis® Express C18 after Supel™ QuE PSA/C18 QuEChERS Cleanup, application for HPLC, application for SPE

G005645

LC/MS/MS of Pesticides in Oranges on Ascentis® Express C18 after Sample Prep using Supel™ Que Z-Sep/C18 QuEChERS sorbent, application for HPLC

G005716

HPLC Analysis of Acetaminophen, Caffeine, Chlorpheniramine, Ibuprofen, Naproxen, Phenylephrine, and Pseudoephedrine on Ascentis® Express C18, application for HPLC

G005882

LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18, application for HPLC

Vorsäulenkartusche

Produkt-Nr.
Beschreibung
Preisangaben

Ähnliches Produkt

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Preisangaben

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317

P-Sätze

P280

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
USMD011246
USMD011245
USMD011244
USMD011243
USMD011242

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Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

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Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Yong Foo Wong et al.
Electrophoresis (2018-02-08)
Metabolic profiling of Glycyrrhiza glabra using comprehensive two-dimensional liquid chromatography (LC × LC) coupled with photodiode array (PDA) and mass spectrometry (MS) detection is described. The separation was conducted under reversed-phase conditions, using a combination of first dimension (1 D) 150 mm microbore
Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
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Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.

Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction

Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.

The Importance of Filtration in Optimizing (U)HPLC and LC-MS Performance

Learn how filter selection and sample and mobile phase filtration impact HPLC back pressure and column lifetime.

Die Bedeutung der Filtration bei der Optimierung der (U)HPLC- und LC/MS-Leistung

Erfahren Sie, wie die Filterauswahl und die Filtration von Proben und mobilen Phasen den HPLC-Gegendruck und die Lebensdauer der Säule beeinflussen.

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Protokolle

Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3

We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.

Chromatograms

application for SPE, application for LC-MSapplication for SPE, application for LC-MSapplication for HPLC, application for SPEapplication for SPE, application for LC-MSMehr anzeigen

Fragen

1-9 von 9 Fragen  

  1. What is the carbon load for Ascentis® Express C18 HPLC Columns?

    1 Antwort

    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared

      Hilfreich?

  2. What is the Department of Transportation shipping information for this product?

    1 Antwort

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  3. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 Antwort

    1. Nothing special is required to use Ascentis Express HPLC columns.  To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.  For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).

      Hilfreich?

  4. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 Antwort

    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      Hilfreich?

  5. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 Antwort

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

      Hilfreich?

  6. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 Antwort

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Hilfreich?

  7. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 Antwort

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      Hilfreich?

  8. Can I use Ascentis Express on a UHPLC system?

    1 Antwort

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

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  9. Can I use Ascentis Express on any type of HPLC system?

    1 Antwort

    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

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