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Sigma-Aldrich

Williams‘ Medium E

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

Medium E, Williams E Medium, Williams’ E medium

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L
glucose: 2.0 g/L
phenol red: 0.0107 g/L
NaHCO3: no

Versandbedingung

ambient

Lagertemp.

2-8°C

Allgemeine Beschreibung

Williams′ E medium is a rich medium developed by Williams′ and Gunn. It is recommended for supporting long-term adult liver epithelial cell culture.

Anwendung

Williams′ E medium has been used in culturing mice/rat primary hepatocytes.

Menge

Formulated to contain 10.8 grams of powder per liter of medium.

Rekonstituierung

Supplement with 2.2 g/L sodium bicarbonate.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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M Herranz-Itúrbide et al.
Redox biology, 40, 101841-101841 (2021-01-26)
Liver is a unique organ in displaying a reparative and regenerative response after acute/chronic damage or partial hepatectomy, when all the cell types must proliferate to re-establish the liver mass. The NADPH oxidase NOX4 mediates Transforming Growth Factor-beta (TGF-β) actions
Christina P Keravnou et al.
Ultrasound in medicine & biology, 42(11), 2676-2686 (2016-08-25)
Localized drug delivery and uptake can benefit from the combined action of ultrasound and microbubbles at a specific site. Some of the possible mechanisms suggested are vessel poration and/or cell poration, but the exact acoustic parameters that trigger those phenomena
Xue Yu et al.
Acta biochimica et biophysica Sinica, 41(12), 1027-1032 (2009-12-17)
Homocysteine is an intermediate in the sulfur amino acid metabolism. Recent studies suggested that there might be links between hyperhomocysteinemia and insulin resistance. In the present study, we investigated the effect of homocysteine on glucose metabolism. We demonstrated that the
D M Toivola et al.
Journal of cell science, 110 ( Pt 1), 23-33 (1997-01-01)
The importance of protein phosphatases in the maintenance of cytoskeletal structure is supported by the serious liver injury caused by microcystin-LR, a hepatotoxic inhibitor of type-1 and type-2A serine/threonine protein phosphatases. We used the microcystin-LR-induced cell injury as a model
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Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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