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T7063

Sigma-Aldrich

Tryptase from human lung

buffered aqueous solution, ≥5 units/mg protein

Synonym(e):

Tryptase enzyme

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About This Item

EC-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Form

buffered aqueous solution

Qualitätsniveau

Spezifische Aktivität

≥5 units/mg protein

Mol-Gew.

~135 kDa

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Tryptase is a glycoprotein released from mast cells during anaphylaxis, which performs a number of functions including catalyzing the activation of complement C3, converting prostromelysin to stromelysin (MMP-3), and cleaving fibrinogen resulting in a loss of clotting potential. Human tryptase is a major secretory protease of human lung mast cells. [1]

Anwendung

Tryptase has been used in a study that purified and characterized recombinant rat mast cell protease 7 expressed in Pichia pastoris. [2] Tryptase has also been used in a study to investigate drug allergies in mast cell disease. [3]

Biochem./physiol. Wirkung

Tryptase is a member of the serine protease S1 family. It is the predominant neutral protease of the mast cell granules. Within the mast cell granule it exists as a heparin-stabilized active tetramer. Stabilization is a result of the high negative charge density of the glycosaminoglycan. This stabilization activity is observed with heparins with a MW greater than 6 kDa as well as other glycosaminoglycans such as dextran sulfate or chondroitin sulfates. Removal of heparin results in dissociation of the tetramer and inactivation of the enzyme. High concentrations of NaCl will result in the dissociation of heparin.
Tryptase is released from the mast cell as a result of the degranulation response during anaphylaxis. In addition, several tryptase genes and alleles (α, β, γ & δ) have been identified in various tissues and circulating in blood. Pro-β-tryptase is thought to be the constituative circulating form in blood.
The biological function of tryptase is unknown. However it has been reported to catalyze the activation of complement C3, convert prostromelysin to stromelysin (MMP-3), and cleave fibrinogen resulting in a loss of clottting potential. Tryptase also degrades fibronectin, calcitonin gene-related peptide, vasoactive intestinal peptide,
and kininogen.

Qualität

Highly purified

Physikalische Eigenschaften

Molecular Weight: ~135 kDa (Human)(Non-covalently linked tetramer with two sets of dissimilar subunits possibly resulting from heterogeneity in N-linked glycosylation and existence of a & b isoforms sequences in human lung). 31-33 kDa (Monomer MW)

Einheitendefinition

One unit will hydrolyze 1.0 μmole of N-benzyl-DL-Arg-pNA per minute at pH 8 at 25 °C.

Physikalische Form

Supplied as a liquid in 50mM Sodium Acetate, 1M Sodium Chloride, pH 5.0 with 0.05mM Heparin and 0.01% Sodium Azide

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Michele Ammendola et al.
Updates in surgery, 65(1), 53-57 (2012-11-03)
Literature data indicate that mast cells (MCs) are involved in angiogenesis through the release of several pro-angiogenetic factors among which tryptase, a serine protease stored in MC granules, is one of the most active. However, no data are available concerning
Liang Chen et al.
Journal of cellular biochemistry, 120(6), 9799-9809 (2018-12-16)
Macrophages polarization plays essential but different roles in most diseases such as atherosclerosis, adipose tissue inflammation, and insulin resistance. Our previous study revealed that protease-activated receptor 2 (PAR2), a G-protein coupled receptor influenced macrophage function, but little is known regarding
Grazyna Michalska-Krzanowska
Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 21(3), 403-408 (2012-12-12)
Determination of serum mast cell tryptase (MCT) is becoming more widely used in diagnosing allergic reactions involving mast cells. It can help evaluate the allergenic effects of drugs administered during anesthesia and the perioperative period. Until now, data about the
Peter Vadas et al.
The Journal of allergy and clinical immunology, 131(1), 144-149 (2012-10-09)
Platelet-activating factor (PAF) is an important mediator and correlates with anaphylaxis severity. How well PAF correlates with severity relative to histamine or tryptase is not known. To analyze the levels of PAF, histamine, and tryptase as a function of severity
Ali Zare-Mirzaie et al.
Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association, 18(5), 322-326 (2012-09-26)
Chronic diarrhea is defined as a decrease in fecal consistency lasting for four or more weeks. Prevalence of this complication in the general population is 5%. Mast cells that play an important role in the regulation of gastrointestinal visceral sensitivity

Fragen

1-2 von 2 Fragen  
  1. What is the extinction coefficient (E1%) of product T7063, Tryptase from human lung?

    1 Antwort
    1. The extinction coefficient (E1%) of product T7063, Tryptase from human lung, is 30.3 when measured at 280 nm wavelength.

      Hilfreich?

  2. What is the Department of Transportation shipping information for this product?

    1 Antwort
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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