SRE0021

Granzyme B human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

• Synonym(e): Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in insect cells
• Assay: ≥90% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: >0.004 units/mg
• Spezifische Aktivität: ≥40 units/μg
• Verpackung: pkg of 5 μg
• UniProt-Hinterlegungsnummer: P10144
• Versandbedingung: wet ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... GZMB(3002)

Beschreibung

Allgemeine Beschreibung

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

Biochem./physiol. Wirkung

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

Einheitendefinition

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl₂.

Physikalische Form

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable