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S6322

Sigma-Aldrich

Sigma Adjuvant System®

oil

Synonym(e):

Adjuvanssystem, Sigma-Adjuvans

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1 VIAL
211,00 €

211,00 €

Versandbereit am14. April 2025Details

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1 VIAL
211,00 €

About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

211,00 €

Versandbereit am14. April 2025Details

Bulk-Bestellung anfordern

Form

oil

Qualitätsniveau

300

Lagertemp.

2-8°C

Anwendung

Adjuvantizitätsassay: EIA-Titer für Testmäuse ist mindestens 2x größer als der von Kontrollmäusen.

Biochem./physiol. Wirkung

Das Sigma Adjuvans-System ist eine stabile Öl-in-Wasser-Emulsion, die als Alternative zu den klassischen Wasser-in-Öl-Emulsionen nach Freund verwendet werden kann. Es wird aus bakteriellen und mykobakteriellen Zellwandbestandteilen gewonnen, die einen potenten Stimulus für das Immunsystem bereitstellen.

Physikalische Form

Jedes Fläschchen enthält 0,5 mg Monophosphoryl-Lipid A (detoxifiziertes Endotoxin) aus Salmonella minnesota und 0,5 mg synthetisches Trehalosedicorynomycolat in 2 % Öl(Squalen)-Tween® 80-Wasser.

Rechtliche Hinweise

Sigma Adjuvant System is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Haftungsausschluss

Sofern in unserem Katalog oder anderen Begleitdokumenten unserer Produkte nicht anders angegeben, sind unsere Produkte nur für Forschungszwecke vorgesehen und nicht für andere Zwecke zu verwenden, einschließlich, jedoch nicht beschränkt auf unautorisierte kommerzielle Verwendung, zur In-vitro-Diagnostik, für Ex-vivo- oder In-vivo-Therapiezwecke oder jegliche Art der Einnahme oder Anwendung bei Menschen oder Tieren.

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H-Sätze

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Gefahreneinstufungen

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000407553
0000407553
0000329924
0000235360
0000235360

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Fragen

1-8 von 8 Fragen  

  1. Is the adjuvant ready to use? I don't see any mention in the datasheet on how to prepare the Antigen:adjuvant mixture. Can you help with that? Thank you.

    1 Antwort

    1. This product requires dilution with saline prior to use. This dilution can be done with or without the addition of the antigen. See below for a brief protocol.

      Prior to antigen addition, warm contents of vial to 40-45 °C. Add 2.0 mL of sterile saline containing the desired amount of antigen.
      The final emulsion will contain a concentration of 2% oil.
      1. Inject antigen-saline solution (2 mL) directly into the vial through the rubber stopper using a syringe fitted with a 20 or 21-gauge needle (leave the cap seal in place).
      2. Vortex the vial vigorously for 2 to 3 minutes to form emulsion. Invert the vial and vortex for 1 minute to ensure reconstitution of any product adhering to the stopper.
      3. If the entire contents of the vial will not be used initially, reconstitute with 1 ml saline without antigen. This emulsion can be stored at 2-8 °C for up to 60 days. Do Not freeze.
      To use, mix aliquots 1:1 with antigen in saline.
      4. Prior to inoculation, warm the vial to 37 °C and vortex briefly.

      For full details on injection protocols and boosting, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

      Hilfreich?

  2. What is the Department of Transportation shipping information for this product?

    1 Antwort

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  3. When using the Sigma Adjuvant System® reagent, Product No. S6322, how do the components MPL and TDM work as an adjuvant?

    1 Antwort

    1. MPL (monophosphoryl lipid A) will bind to CD14 on the cell surface similar to LPS.  TDM (trehalose dicorynomycolate) is an analog of one of the components of the tubercle bacillus. Both of these components act like a superantigen and activate the immune system nonspecifically.  This allows for a greater response to the immunogen delivered in this adjuvant.

      Hilfreich?

  4. Can you reconstitute and store the Sigma Adjuvant System® reagent (Product No. S6322) if not using the entire vial right away?

    1 Antwort

    1. If the entire contents of the vial will not be used initially, reconstitute with 1 ml saline without antigen.  This emulsion can then be stored at 2-8 °C for up to 60 days.  Do Not Freeze. To use, mix aliquots 1:1 with antigen in saline. Prior to inoculation, warm the vial to 37 °C and vortex briefly.  This and other information can be found on the Sigma Adjuvant System® reagent (Product No. S6322) Product Information Sheet.

      Hilfreich?

  5. What is used to solubilize Product No. S6322, Sigma Adjuvant System® reagent?

    1 Antwort

    1. The Sigma Adjuvant System® reagent (Product No. S6322) can be solubilized in a sterile saline solution containing the desired amount of antigen to a final volume of 2 ml.  For more details, please see the S6322 Product Information Sheet.

      Hilfreich?

  6. Is the Sigma Adjuvant System®, Product No S6322, the same as the RIBI adjuvant?

    1 Antwort

    1. Yes, the Sigma Adjuvant System®, Product No S6322, is also called RIBI adjuvant.

      Hilfreich?

  7. Has Sigma Adjuvant System®, Product S6322, been used to induce EAE (Experimental autoimmune encephalomyelitis)?

    1 Antwort

    1. Sigma Adjuvant System®, Product S6322 is a replacement for MPT+TDM adjuvant (Ribi, Product M6536) that was discontinued by the supplier Corixa.  Although we have not tested Product S6322 for induction of Experimental autoimmune encephalomyelitis (EAE), the following reference cites the use of MBP in Ribi adjuvant for induction of acute EAE.The Journal of ImmunologyOctober 15, 2005, 175(8): 5077-5086

      Hilfreich?

  8. When using the Sigma Adjuvant System®, Product No. S6322, how is the antigenic response boosted?

    1 Antwort

    1. With the Sigma Adjuvant System® (Product No. S6322) with mice, rabbits, rats and guinea pigs; boost on day 21 or 28, bleed 10 - 14 days after booster injection. Boost every 3 - 4 weeks thereafter. For goats; boost every four weeks. Use the same protocol for each injection.  For more information please see the Product Information Sheet for Product No. S6322.

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