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R6501
Ribonuclease H from Escherichia coli
recombinant, expressed in E. coli, buffered aqueous glycerol solution, 1,000-4,000 units/mL
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About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54
Empfohlene Produkte
Rekombinant
expressed in E. coli
Form
buffered aqueous glycerol solution
Mol-Gew.
17.6 kDa
Verpackung
vial of ~30 units
Konzentration
1,000-4,000 units/mL
Versandbedingung
dry ice
Lagertemp.
−20°C
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Anwendung
Einheitendefinition
One unit hydrolyzes 1.0 nanomole RNA in 3H-labeled poly(A) • poly(dT) to acid soluble material in 20 min at 37 °C.
Physikalische Form
Solution in 50% glycerol containing 20 mM Tris-HCl, pH 7.5, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT and 0.05 mg BSA per ml
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Ribonuclease H (Escherichia coli) contains one strong magnesium-binding site, as determined by metal-titration experiments monitored by high field 1H-NMR and also by direct titration calorimetry. Kinetic and thermodynamic parameters were evaluated by 25Mg-NMR and were as follows: dissociation constant Kd
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