R0508

(−)-Riboflavin

acrylamide photopolymerization tested

• Synonym(e): Laktoflavin, Vitamin B₂, Vitamin G
• Empirische Formel (Hill-System): C₁₇H₂₀N₄O₆
• CAS-Nummer: 83-88-5
• Molekulargewicht: 376.36
• Beilstein: 97825
• EG-Nummer: 201-507-1
• MDL-Nummer: MFCD00005022
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 57654584

Eigenschaften

• Biologische Quelle: synthetic (organic)
• Form: powder
• mp (Schmelzpunkt): 290 °C (dec.) (lit.)
• Eignung: acrylamide photopolymerization tested
• Fremdaktivität: Protease, none detected
• SMILES String: CC1=C(C)C=C(N(C[C@H](O)[C@@H]([C@H](O)CO)O)C(C2=N3)=NC(NC2=O)=O)C3=C1
• InChI: 1S/C17H20N4O6/c1-7-3-9-10(4-8(7)2)21(5-11(23)14(25)12(24)6-22)15-13(18-9)16(26)20-17(27)19-15/h3-4,11-12,14,22-25H,5-6H2,1-2H3,(H,20,26,27)/t11-,12+,14-/m0/s1
• InChIKey: AUNGANRZJHBGPY-SCRDCRAPSA-N

Beschreibung

Anwendung

Riboflavin is suitable as a photopolymerization reagent in PAGE by forming free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. Riboflavin is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)