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P5368

Sigma-Aldrich

Phosphatgepufferte Kochsalzlösung -Lösung

powder, pH 7.4, BioPerformance Certified, for molecular biology

Synonym(e):

PBS

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10 PKG
118,00 €

118,00 €


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10 PKG
118,00 €

About This Item

MDL-Nummer:
UNSPSC-Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

118,00 €


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Produktbezeichnung

Phosphatgepufferte Kochsalzlösung -Lösung, BioPerformance Certified, pH 7.4

Qualität

BioPerformance Certified
for molecular biology

Qualitätsniveau

Form

powder

Lagerbedingungen

(Keep container tightly closed in a dry and well-ventilated place.)

Methode(n)

immunofluorescence: suitable

Verunreinigungen

BioBurden, tested
endotoxin, tested

Farbe

white

pH-Wert

7.4

Löslichkeit

water: 9.07-10.0 g/L, clear, colorless

Kationenspuren

Fe: ≤10 ppm
heavy metals (as Pb): ≤5 ppm

Eignung

suitable for SDS-PAGE
suitable for molecular biology

Anwendung(en)

diagnostic assay manufacturing
histology
sample preparation

Fremdaktivität

DNase, RNase, Protease, none detected

SMILES String

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChIKey

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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Anwendung


  • Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: In dieser Studie wurde phosphatgepufferte Kochsalzlösung (PBS) verwendet, um eine kostengünstige und effiziente Methode zur DNA-Extraktion aus Insekten zu entwickeln, die routinemäßige PCR-basierte Anwendungen in der Molekularbiologie erleichtert (Jangra & Ghosh, 2022).

  • Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: PBS wurde verwendet, um aus Fettgewebe stammende Stammzellen in einem injizierbaren Hydrogel zu präparieren und zu verabreichen, was Potenzial für innovative Osteoarthritis-Behandlungen zeigt (Bhattacharjee et al., 2022).

  • Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: In dieser Forschungsarbeit wurde PBS für die Probenvorbereitung in einer neuartigen LC-MS-Methode zum Nachweis von SARS-CoV-2 verwendet, die ein schnelles und sensitives Diagnosetool bietet (Hober et al., 2021).

Verpackung

Folienbeutel

Rekonstituierung

Der Inhalt eines Beutels, in einem Liter destilliertem oder entionisiertem Wasser gelöst, ergibt eine 0,01 M phosphatgepufferte Kochsalzlösung (NaCl – 0,138 M; KCl – 0,0027 M), pH 7,4 bei 25 °C.

Sonstige Hinweise

Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

Jesús Gilberto Arámburo-Galvez et al.
Nutrients, 10(7) (2018-07-18)
Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability of some foods to sensitize and trigger allergic reactions. However, there are no validated animal models to evaluate the sensitizing
Petra J Roubos-van den Hil et al.
Veterinary microbiology, 202, 58-63 (2016-09-27)
Enterotoxigenic E. coli (ETEC), causing post-weaning diarrhoea, is a major problem in weaned piglets. Individual animal responses to ETEC infection show high variability in animal experiments. Two studies were designed to optimize the ETEC F4ac infection model in piglets by
Jinjin Sun et al.
Nanoscale research letters, 14(1), 248-248 (2019-07-26)
Herein, a magnetic graphene field-effect transistor biosensor was prepared through the transfer of a chemical vapor deposition graphene film onto a glass substrate to produce a sensing film and conductive channel. By fixing 1-pyrenebutanoic acid succinimidyl ester onto graphene film
Yixi Chen et al.
The European journal of neuroscience, 49(4), 510-524 (2018-11-26)
An emerging treatment for Parkinson's disease (PD) is cell replacement therapy. Authentic midbrain dopaminergic (mDA) neuronal precursors can be differentiated from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). These laboratory-generated mDA cells have been demonstrated
Gary Burness et al.
Biology open, 7(4) (2018-04-06)
Female birds transfer maternally derived antibodies (matAb) to their nestlings, via the egg yolk. These antibodies are thought to provide passive protection, and allow nestlings to avoid the costs associated with mounting an innate immune response. To test whether there

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Fragen

1-5 von 5 Fragen  
  1. What is the shelf-life of product P5368, phosphate buffered saline (PBS)?

    1 Antwort
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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  2. What is the Department of Transportation shipping information for this product?

    1 Antwort
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  3. Does Product P5368, Phosphate buffered saline (PBS), contain calcium and magnesium?

    1 Antwort
    1. No.  The complete formulation is shown in the product listing.  When reconstituted, the resulting solution is 10 mM phosphate buffer, with 138 mM sodium chloride and 2.7 mM potassium chloride.

      Hilfreich?

  4. What volume of Product P5368, Phosphate buffered saline (PBS), does each pack make?

    1 Antwort
    1. When the contents of a pouch is dissolved in 1 Liter of water, it makes 1X PBS, pH 7.4 at 25°C.

      Hilfreich?

  5. If reconstituted with sterile water, will Product P5368, Phosphate buffered saline (PBS), be sterile?

    1 Antwort
    1. No.  To make this PBS sterile, the solution should be either sterile-filtered or autoclaved.

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