L2650
Lektin aus Glycine max (Sojabohne)
peroxidase conjugate, lyophilized powder
Synonym(e):
Sojabohnen Agglutinin
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About This Item
UNSPSC-Code:
12352202
NACRES:
NA.32
Empfohlene Produkte
Biologische Quelle
Glycine max (soybean)
Konjugat
peroxidase conjugate
Assay
85.00-100.00% (Warburg-Christian)
Form
lyophilized powder
Wirksamkeit
<80 μg per mL agglutination activity
peroxidase activity
25-100 units/mg protein
Zusammensetzung
Protein, ~95% modified Warburg-Christian
Methode(n)
western blot: suitable
UniProt-Hinterlegungsnummer
Lagertemp.
−20°C
Angaben zum Gen
soybean ... LE1(100818710)
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Anwendung
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem./physiol. Wirkung
SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.
Einheitendefinition
1 U bildet 1 mg Purpurogallin in 20 Sek. aus Pyrogallol bei pH 6.0 und 20 °C.
Physikalische Form
Enthält Natriumcitrat
Angaben zur Herstellung
Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan,[1] which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Hinweis zur Analyse
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.
Lagerklassenschlüssel
13 - Non Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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