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Merck

H6024

Sigma-Aldrich

Hoechst Stain solution

Synonym(e):

Bisbenzimide H 33258

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About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352207
PubChem Substanz-ID:
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Form

solution

Anwendung(en)

histology

Lagertemp.

2-8°C

SMILES String

O.Cl.Cl.Cl.CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6

InChI

1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2

InChIKey

OWRSPPSBNWJJAR-UHFFFAOYSA-N

Allgemeine Beschreibung

Hoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic preparations and in unfixed cultured cells.[1] It can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. Hoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication.[2]

Anwendung

A fluorescent DNA stain qualified for use in Mycoplama staining.

Komponenten

Contains 0.5μg/ml Hoechst bisbenzimide 33258 fluorochrome stain and thimerosal.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Marisa Müller et al.
PLoS biology, 9(4), e1000611-e1000611 (2011-04-29)
In eukaryotes, hundreds of mRNAs are localized by specialized transport complexes. For localization, transcripts are recognized by RNA-binding proteins and incorporated into motor-containing messenger ribonucleoprotein particles (mRNPs). To date, the molecular assembly of such mRNPs is not well understood and
Feifan Zhou et al.
International journal of cancer, 125(6), 1380-1389 (2009-06-18)
Although anti-tumor immunological responses have been mainly associated with necrosis, apoptosis-associated immune responses have been recently suggested as well. In this study, we investigated anti-tumor immune responses and regulatory mechanisms of HSP70 using apoptotic cells induced by photodynamic therapy (PDT).
Kin Lam Fok et al.
Endocrinology, 158(11), 4000-4016 (2017-09-25)
Spermatogenesis is sustained by a heterogeneous population of spermatogonia that includes the spermatogonial stem cells. However, the mechanisms underlying their establishment from gonocyte embryonic precursors and their maintenance thereafter remain largely unknown. In this study, we report that inactivation of
Nicola Cirillo et al.
British journal of cancer, 117(7), 984-993 (2017-08-11)
Recent studies have shown that production of cortisol not only takes place in several non-adrenal peripheral tissues such as epithelial cells but, also, the local inter-conversion between cortisone and cortisol is regulated by the 11β-hydroxysteroid dehydrogenases (11β-HSDs). However, little is
S A Latt et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 23(7), 493-505 (1975-07-01)
A number of applications of the detection of deoxyribonucleic acid synthesis by fluorescence microscopy are illustrated. These include (a) the analysis of sister chromatid exchanges and sister chromatid segregation at mitosis, (b) the location of chromosome regions containing deoxyribonucleic acid

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