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G1270

Sigma-Aldrich

L-Glutamine Synthetase from Escherichia coli

lyophilized powder, 400-2,000 units/mg protein

Synonym(e):

L-Glutamate:ammonia ligase (ADP-forming)

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About This Item

CAS-Nummer:
9023-70-5
EC-Nummer:
6.3.1.2 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00131463
UNSPSC-Code:
12352204
NACRES:
NA.26

Form

lyophilized powder

Qualitätsniveau

200

Spezifische Aktivität

400-2,000 units/mg protein

Aufgereinigt durch

affinity chromatography

Enthält

dithioerythritol as preservative

Zusammensetzung

Protein, ~5% Lowry

Löslichkeit

H2O: soluble 0.95-1.05 mg/mL, clear to hazy

UniProt-Hinterlegungsnummer

P0A9C5

Fremdaktivität

ATPase <0.2%

Lagertemp.

−20°C

Angaben zum Gen

Escherichia coli K12 ... glnA(948370)

Allgemeine Beschreibung

L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.

Anwendung

L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

Biochem./physiol. Wirkung

L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.
Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.
Degradative enzyme for glutamic acid

Einheitendefinition

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

Physikalische Form

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Reversible Adenylylation of Glutamine Synthetase Is Dynamically Counterbalanced during Steady-State
Okano H, et al.
Journal of molecular biology, 404(1), 522-536 (2010)
Structural Analysis of Glutamine Synthetase from Helicobacter pylori
Joo HK, et al.
Scientific Reports, 8(1), 11657-11657 (2018)
Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation
Jin DY, et al.
The Journal of Biological Chemistry, 272(49), 30952-30961 (1997)
Ammonia acquisition in enteric bacteria: physiological role of the ammonium/methylammonium transport B (AmtB) protein
Soupene E, et al.
Proceedings of the National Academy of Sciences of the USA, 95(12), 7030-7034 (1998)
Kamal Krishna Singh et al.
Plant cell reports, 32(2), 183-193 (2012-10-17)
KEY MESSAGE : The regulation of GS isoforms by WD was organ specific. Two GS isoforms i.e. OsGS1;1 and OsGS2 were differentially regulated in IR-64 (drought-sensitive) and Khitish (drought-tolerant) cultivars of rice. Water deficit (WD) has adverse effect on rice
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