D9281

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

• UNSPSC-Code: 41141827
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 100; 200
• Form: liquid
• Verwendung: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• Eignung: suitable for electrophoresis (DNA)
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Anwendung

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.[1]

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Komponenten

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Sonstige Hinweise

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Angaben zur Herstellung

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317
• P-Sätze: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Gefahreneinstufungen: Skin Sens. 1
• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable