T4415

Tris-Borat-EDTA-Puffer

BioReagent, suitable for electrophoresis, 10× concentrate

• Synonym(e): TBE buffer
• MDL-Nummer: MFCD00236358
• UNSPSC-Code: 41105319
• PubChem Substanz-ID: 329826793
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 200
• Produktlinie: BioReagent
• Form: solution
• Verunreinigungen: DNase and RNase, none detected
• Eignung: suitable for electrophoresis
• SMILES String: OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
• InChIKey: OSBLTNPMIGYQGY-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Anwendung

Ready for use in gel electrophoresis after dilution to working concentrations.
Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Verpackung

The 4L, 10L and 20L sizes are supplied in dispenser with a spigot.

Angaben zur Herstellung

Prepared with 18 megohm water

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Sonstige Hinweise

TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H360FD
• P-Sätze: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Gefahreneinstufungen: Repr. 1B
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type ABEK (EN14387) respirator filter