D0672

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

• NACRES: NA.25
• UNSPSC Code: 41105335
• Form: liquid
• Storage temp.: −20°C

Eigenschaften

• Quality Segment: 100, 200
• form: liquid
• usage: 200 uses (0.1 mg size), 40 uses (20 μg size)
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Beschreibung

General description

Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Preparation Note

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)