C0743

Anti-Calsequestrin-1 (N-terminal) in Kaninchen hergestellte Antikörper

affinity isolated antibody

• Synonym(e): Anti-CALMITINE, Aspartactin, CASQ1, Calsequestrin- skeletal muscle isoform
• MDL-Nummer: MFCD09265356
• UNSPSC-Code: 12352203
• NACRES: NA.44

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~60 kDa
• Speziesreaktivität: rat, mouse, bovine (predicted), human (predicted)
• Konzentration: ~1.0 mg/mL
• Methode(n): western blot: 0.5-1 μg/mL using extract of rat skeletal muscle S1 fraction
• UniProt-Hinterlegungsnummer: P31415
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CASQ1(844) ; mouse ... Casq1(12372)

Beschreibung

Immunogen

synthetic peptide corresponding to amino acids 28-45 located near the N-terminus of mouse calsequestrin-1, conjugated to KLH. This sequence is identical in rat and highly conserved in human and bovine calsequestrin-1 (two amino acid substitution). This sequence is not found in calsequestrin-2.

Anwendung

Anti-Calsequestrin-1 (N-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using extracts of rat skeletal muscle S1 fraction.

Biochem./physiol. Wirkung

Calsequestrin (CS) is a calcium-binding protein in the sarcoplasmic reticulum (SR). It is known as CSQ and PDIB1. The gene belongs to calsequestrin protein family. Calsequestrin-1 is a putative auto-antigen associated with eye muscle inflammation in Graves′ disease. Calsequestrin-2 (55 kDa) is found in the SR′s terminal cisternae luminal space of cardiac, slow skeletal muscle cells and may be in platelets. The major Ca2+ binding protein in cardiac and skeletal muscle is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the SR. It helps in controlling the smooth-muscle contraction.

Zielbeschreibung

Calsequestrin (CS also known as CSQ), the major Ca²⁺ binding protein in cardiac and skeletal muscle, is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which functions as an internal Ca²⁺ store in the lumen of the SR. In mammalians, two forms of the protein are found, calsequestrin-1 (CASQ-1) and calsequestrin-2 (CASQ-2), which encode the fast-twitch skeletal muscle and cardiac calsequestrin, respectively. Calsequestrin-1 is found in the SRs terminal cisternae luminal space of fast skeletal muscle cells. Calsequestrin-1 is a putative autoantigen associated with eye muscle inflammation in Graves′ disease.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)