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B6916

Supelco

Bradford-Reagenz

Bradford Reagent

for 0.1-1.4 mg/ml protein

Synonym(e):

Coomassie dye binding protein assay, Protein dye reagent

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500 ML
154,00 €

154,00 €

Voraussichtliches Versanddatum02. April 2025

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500 ML
154,00 €

About This Item

UNSPSC-Code:
12161500
NACRES:
NA.32

154,00 €

Voraussichtliches Versanddatum02. April 2025

Bulk-Bestellung anfordern

Qualitätsniveau

200

Form

solution

Lagertemp.

2-8°C

Allgemeine Beschreibung

Bei dem Bradford-Assay wird der Proteinlösung Coomassie-Brillantblau G-250 hinzugefügt. Der Farbstoff Coomassie-Brillant-Blau lagert sich an basische und aromatische Aminosäuren an und verursacht damit bei der Proteinbestimmung eine Verschiebung in der Absorption.[1]

Anwendung

Das Bradford-Reagenz wird zur Bestimmung der gesamten Proteinkonzentration verwendet.[2][3][4]

Leistungsmerkmale und Vorteile

  • Das Reagenz ist gebrauchsfertig. Kein Mischen bzw. keine Verdünnung erforderlich.
  • Die Farbentwicklung geht schnell vonstatten. Nach nur fünf Minuten Inkubation wird die Probe bei 595 nm abgelesen.
  • Reduzierende Zucker und reduzierende Substanzen zusammen mit Thiolen beeinträchtigen dieses Reagenz nicht.
  • Das Reagenz eignet sich für Mikro-Assays (1 - 10 μg/ml) und Standard-Assays (50 - 1400 μg/ml).
  • Es kann bei Mikrotiterplatten-Assays verwendet werden.
  • Kostengünstiger Assay.

Sonstige Hinweise

Gewinner des CiteAb Award 2024 in der Kategorie „Supplier Succeeding in Parkinson′s Research“ (Erfolgreiche Anbieter in der Parkinson-Forschung)

Rechtliche Hinweise

Anwendung

Produkt-Nr.
Beschreibung
Preisangaben

FP0010

FluoroProfile™ Protein-Bestimmungs-Kit

auch häufig zusammen mit diesem Produkt gekauft

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TP0100

Gesamtprotein-Kit, Micro

Piktogramme

Health hazardCorrosion
GHS08,GHS05

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

Zielorgane

Eyes,Central nervous system

Lagerklassenschlüssel

8B - Non-combustible corrosive hazardous materials

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

EU REACH Annex XVII (Restriction List)

CAS No.

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000261841
0000261841
SLCN3585
SLCK1933
SLCG1879

Die passende Version wird nicht angezeigt?

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Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Sin-Jin Li et al.
Clinical nutrition (Edinburgh, Scotland), 36(3), 760-767 (2016-06-28)
The cellular mechanisms of obesity-induced cardiomyopathy are multiple and not completely elucidated. The objective of this study was to differentiate two obesity-associated cardiomyopathy miniature pig models: one with the metabolic syndrome (MetS), and one with a metabolically healthy obesity (MHO).
Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Charoenwong D et al.
Applied and Environmental Microbiology, 77, 5220-5229 (2011)
Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations.
Czebe K et al.
Respiratory Medicine, 102, 720-720 (2008)
M Tal et al.
The Journal of biological chemistry, 260(18), 9976-9980 (1985-08-25)
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their
Proteomic response of the biological control fungus Trichoderma atroviride to growth on the cell walls of Rhizoctonia solani.
Grinyer J et al.
Current Genetics, 47, 381-381 (2005)
Alle zugehörigen Dokumente anzeigen

Artikel

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

Protokolle

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Verwandter Inhalt

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Proteinquantifizierung

Proteinquantifizierungsmethoden, Reagenzien und Immunassaytechnologie für die präzise Messung der Proteinkonzentration in unterschiedlichen Proben.

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Fragen

1-10 von 11 Fragen  

  1. Talking about the Bradford reagent, I read that: The linear concentration range is 0.1-1.4 mg/mL of protein... does this mean I can prepare these concentrations of protein which will then be diluted when mixed with a certain amount of reagent?

    1 Antwort

    1. For the Standard 3.1 mL Assay and 96 Well Plate Assay, the protein standards should be prepared in the range of 0.1 - 1.4 mg/mL. This is not the final concentration of the protein after being diluted in the assay.

      For the full protocol, please refer to the document found here:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/165/479/b6916bul-ms.pdf

      Hilfreich?

  2. Hallow, Does Bradford Reagent B6916 have color? I don't see it the SDS

    1 Antwort

    1. The color of this product can range from Faint Yellow-Brown to Light Brown. The exact result will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’: https://www.sigmaaldrich.com/product/sigma/b6916#product-documentation

      Hilfreich?

  3. Does this product (B6916) suitable for use with buffers with 1% SDS?

    1 Antwort

    1. A compatibility chart is listed in the bulletin. This chart indicates that this reagent is compatible with concentrations of SDS up to 0.125%.

      Hilfreich?

  4. Can i use this reagent to quantify protein range from 0-10 ug? May i dilute this bradford reagent, and if i do, will it affect the accuracy of the reading?

    1 Antwort

    1. The Bradford method has a lower limit of detection of 20 ug/mL. The Lowry method has a lower limit of 10 ug/mL. Concentration of the sample may be necessary.
      See the links below for additional helpful information:

      Protein Quantitation Methods-
      https://www.sigmaaldrich.com/applications/protein-biology/protein-quantitation

      Amicon Centrifugal Filters-
      https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/amicon-ultra-centrifugal-filters

      Centricon Centrifugal Filters-
      https://www.sigmaaldrich.com/substance/centriconplus70centrifugalfilter1234598765

      Hilfreich?

  5. How long is the  reagant stable or can it separate and require mixing prior to use? 

    1 Antwort

    1. Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of BSA is very good (especially if the solutions are stored as frozen aliquots). In fact, albumins are frequently used as stabilizers for other solubilized proteins (e.g., labile enzymes). However, albumin is readily coagulated by heat. When heated to 50°C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. At somewhat lower temperatures aggregation is also expected to occur, but at relatively slower rates.

      Please see the product data sheet which describes the solution stability of BSA:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/351/531/a8412pis.pdf

      Hilfreich?

  6. When using Product B6916, Bradford Reagent, how soon after my color development do I need to read my assay?

    1 Antwort

    1. he protein-dye complex is stable up to 60 minutes. The absorbency of the samples must be recorded before the 60 minute time limit and within 10 minutes of each other.

      Hilfreich?

  7. Will the buffer or solution my protein is in interfere with Product B6916, Bradford Reagent?

    1 Antwort

    1. A compatibility chart is listed in the bulletin. If your substance is not listed, then we recommend testing this by diluting the standard protein samples in the same buffer as the unknown samples.

      Hilfreich?

  8. When using Product B6916, Bradford Reagent, what can I do if I have a very dilute sample in a large volume?

    1 Antwort

    1. The micro assay using this same reagent may be an option for you. The micro assay is used when a large volume (at least 1 mL) of a dilute sample is available for testing. The linear concentration range of this assay is lower than the standard or multiwell plate assays, (1-10 μg of total protein in 1 mL).

      Hilfreich?

  9. What is the Department of Transportation shipping information for this product?

    1 Antwort

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  10. What is the useful concentration range that can be measured by Product B6916, Bradford Reagent?

    1 Antwort

    1. The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.

      Hilfreich?

1-10 von 11 Fragen  

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