1.15444

Coomassie Brilliant blue G 250 (C.I. 42655)

for electrophoresis

• Synonym(e): Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain
• Empirische Formel (Hill-System): C₄₇H₄₈N₃NaO₇S₂
• CAS-Nummer: 6104-58-1
• Molekulargewicht: 854.02
• MDL-Nummer: MFCD00078482
• UNSPSC-Code: 41105305
• EG-Index-Nummer: 228-058-4
• NACRES: NC.01

Eigenschaften

• Qualitätsniveau: 100
• Wirksamkeit: >5000 mg/kg LD₅₀, oral (Rat)
• Methode(n): protein staining: suitable
• pH-Wert: 6.4 (20 °C, 10 g/L in H₂O)
• Löslichkeit: 40 g/L
• Schüttdichte: 520 kg/m³
• Lagertemp.: no temp limit
• InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1
• InChIKey: CMGWKYXJIYYESN-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.

Anwendung

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It has also been used in Bradford protein assay.

Hinweis zur Analyse

Identität (UV/VIS-Spektrum): entspricht
Absorptionsmaximum λ_max. (Puffer pH 7.0): 577 - 584 nm
Spez. Extinktion E1%/1cm (λ_max; 0.01 g/l; Puffer pH 7.0; berechnet auf getrocknete Substanz): 450 - 570
DC-Prüfung: entspricht
Trocknungsverlust (110 °C): ≤ 8 %
Eignung für die Elektrophorese: entspricht

Rechtliche Hinweise

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable