B0631

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt

≥90%

• Synonym(e): 5-BrdUTP
• Empirische Formel (Hill-System): C₉H₁₄BrN₂O₁₄P₃
• CAS-Nummer: 102212-99-7
• Molekulargewicht: 547.04
• MDL-Nummer: MFCD00057405
• UNSPSC-Code: 41106305
• PubChem Substanz-ID: 24891527
• NACRES: NA.51

Eigenschaften

• Biologische Quelle: synthetic (organic)
• Assay: ≥90%
• Form: powder
• Löslichkeit: water: 50 mg/mL, clear, colorless to faintly yellow
• Lagertemp.: −20°C
• SMILES String: [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H14BrN2O14P3.Na.H/c10-4-2-12(9(15)11-8(4)14)7-1-5(13)6(24-7)3-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h2,5-7,13H,1,3H2,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• InChIKey: UWZGQOWZUSBZMC-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

5-Bromo-2′-deoxyuridine 5′-triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles.

Anwendung

5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:

• in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cells
• to label cleaved DNA ends in apoptosis assay in avian tissue sections
• for incorporation into mice prostates for immunohistochemistry studies

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges