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646563

Supelco

DL-Dithiothreitol -Lösung

1 M in H2O

Synonym(e):

(±)-threo-1,4-Dimercapto-2,3-butandiol -Lösung, DTT

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10 X 0.5 ML
114,00 €

114,00 €

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10 X 0.5 ML
114,00 €

About This Item

Empirische Formel (Hill-System):
C4H10O2S2
CAS-Nummer:
3483-12-3
Molekulargewicht:
154.25
Beilstein:
1719757
MDL-Nummer:
MFCD00004877
UNSPSC-Code:
12161504
PubChem Substanz-ID:
24883472
NACRES:
NA.25
Form:
liquid

114,00 €

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Form

liquid

Qualitätsniveau

100

Eignung der Reaktion

reagent type: reductant

Konzentration

1 M in H2O

Dichte

1.04 g/mL at 20 °C
1.035 g/mL at 25 °C

SMILES String

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChIKey

VHJLVAABSRFDPM-QWWZWVQMSA-N

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Allgemeine Beschreibung

Dithiothreitol (DTT) wird in Proteomikanwendungen eingesetzt, um die Sulfhydrylgruppen (-SH) im reduzierten Zustand zu erhalten sowie für die quantitative Reduktion von Disulfidgruppen (-S-S-). Es ist ein häufig in Puffern eingesetztes Reagens, da es die Oxidationsstufe einer Proteinprobe reduzieren und die Enzymaktivität erhalten kann. DTT wird bei der Reduktion von anderen Disulfiden in Lösung zu zyklischem Disulfid oxidiert.
Dithiothreitol ist ein wirksames Reduktionsmittel für die Proteinanalyse. Dieses versiegelte Ampullenformat ist praktisch und leicht zu verwenden, wenn Proben für die Elektrophorese oder andere Anwendungen hergestellt werden, und vermeidet Abfall sowie einen Potenzverlust.

Anwendung

DL-Dithiothreitol-Lösung wird wie folgt verwendet:
  • In der Western-Blot-Analyse
  • als Ergänzung zu Lysepuffer zum Untersuchen ihrer Wirkung auf die Spermienlyse
  • als Komponente von kaltem Homogenisierungspuffer und gekühltem Waschpuffer für die Isolierung von Zellkernen aus gefrorenem Rattengewebe
  • in SDS-PAGE, Chromatografie, Modifikation von Cystein-haltigen Verbindungen

Leistungsmerkmale und Vorteile

Niedrige Toxizität und weniger Geruch im Vergleich zu anderen Thiolverbindungen.

Zubehör

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Corrosion
GHS05

Signalwort

Danger

H-Sätze

H315,H318

Gefahreneinstufungen

Eye Dam. 1 - Skin Irrit. 2

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
MKCW8841
MKCV9188
MKCV8762
MKCW6078
MKCV6891

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L-(−)-Dithiothreitol ≥95% (titration)

Sigma-Aldrich

D9760

L-(−)-Dithiothreitol

Jodacetamid Single use vial of 56 mg

Sigma-Aldrich

A3221

Jodacetamid

Sputolysin® Reagenz Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

Millipore

560000

Sputolysin® Reagenz

DTT OmniPur® Grade, >= 99.4%, Used for reduction of protein disulfide linkages

Millipore

3860-OP

DTT

Willem-Jan M Schellekens et al.
Critical care (London, England), 18(1), R28-R28 (2014-02-11)
Diaphragm weakness induced by prolonged mechanical ventilation may contribute to difficult weaning from the ventilator. Hypercapnia is an accepted side effect of low tidal volume mechanical ventilation, but the effects of hypercapnia on respiratory muscle function are largely unknown. The
Calista K L Ng et al.
Human molecular genetics, 24(11), 3163-3171 (2015-02-26)
mRNA decay is an essential and active process that allows cells to continuously adapt gene expression to internal and environmental cues. There are two mRNA degradation pathways: 3' to 5' and 5' to 3'. The DCPS protein is the scavenger
Yukari Yamane-Sando et al.
MicrobiologyOpen, 3(2), 196-212 (2014-02-11)
Sphingolipids are a family of eukaryotic lipids biosynthesized from sphingoid long-chain bases (LCBs). Sphingolipids are an essential class of lipids, as their depletion results in cell death. However, acute LCB supplementation is also toxic; thus, proper cellular LCB levels should
I Dzneladze et al.
Leukemia, 29(7), 1485-1495 (2015-03-05)
In this study, we investigated the role of inositol polyphosphate-4-phosphatase, type-II (INPP4B) in acute myeloid leukemia (AML). We observed that AML patients with high levels of INPP4B (INPP4B(high)) had poor response to induction therapy, shorter event-free survival and shorter overall
Kazuya Ishikawa et al.
Nature communications, 5, 5430-5430 (2014-11-13)
Pathogen effector proteins are delivered to host cells to suppress plant immunity. However, the mechanisms by which effector proteins function are largely unknown. Here we show that expression of XopP(Xoo), an effector of rice pathogen Xanthomonas oryzae pv. oryzae, in
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Fragen

1-6 von 6 Fragen  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 Antwort

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 Antwort

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Hilfreich?

  3. after opening the ampule is DTT still usable or only single use?

    1 Antwort

    1. This product is intended for single use. The stability of the solution after opening the ampule has not been determined. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/629/609/646563dat.pdf

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  4. how to use DDT

    1 Antwort

    1. 646563 (DL-Dithiothreitol solution, DTT) could be applied for research, such as western blot analysis, lysis buffer, etc. The procedure of SDS-PAGE sample preparation with DTT has been listed in the datasheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/629/609/646563dat.pdf
      For other applications, please check the "Peer Reviewed Papers" section on the web for more literature: https://www.sigmaaldrich.com/product/sigma/646563

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  5. What is the expiration time for this product?

    1 Antwort

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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  6. What are the storage conditions for this product?

    1 Antwort

    1. This product is stored in room temperature. Store tightly closed in a dry and well-ventilated place. Please see section 7 of the Safety Data Sheet for more information: https://www.sigmaaldrich.com/sds/sigma/646563

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