MAB1397Z

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free

clone 3E2B, Chemicon^®, from hamster(Armenian)

• Synonym(e): CD54, MAB1397
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: hamster (Armenian)
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 3E2B, monoclonal
• Speziesreaktivität: mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NM_000201.1
• UniProt-Hinterlegungsnummer: P05362
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Icam1(15894)

Beschreibung

Spezifität

MAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Adhäsions-Proteine (CAMs – Zelladhäsionsmoleküle)

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH.

Immunocytochemistry: Live or acetone fixed cells

ELISA

Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).

Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.

Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary

Clone 3E2B is reported to be function blocking against murine ICAM-1.

Optimal working dilutions must be determined by end user.

Verlinkung

Replaces: CBL1331F

Physikalische Form

Format: Purified

Purified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg

Lagerung und Haltbarkeit

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable