233156

Cleland′s Reagent, Molecular Biology Grade

A protective agent for SH groups.

• Synonym(e): Cleland’s Reagent, Molecular Biology Grade, 1,4-Dithiothreitol, DTT
• Empirische Formel (Hill-System): C₄H₁₀O₂S₂
• CAS-Nummer: 3483-12-3
• Molekulargewicht: 154.25
• MDL-Nummer: MFCD00004877
• UNSPSC-Code: 12161504
• NACRES: NA.25
• Form: powder; solid
• Assay: ≥99% (titration)

Eigenschaften

• Qualität: Molecular Biology
• Qualitätsniveau: 100
• Assay: ≥99% (titration)
• Form: powder; solid
• Eignung der Reaktion: reagent type: reductant
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Farbe: white
• Löslichkeit: water: 15 mg/mL
• Kationenspuren: heavy metals: ≤0.001%
• Absorption: ≤0.100 at 280 nm at 100 mM (1 cm); ≤0.500 at 260 nm at 100 mM (1 cm)
• Fremdaktivität: DNases, proteases, RNases, none detected
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• SMILES String: SC[C@H](O)[C@@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• InChIKey: VHJLVAABSRFDPM-IMJSIDKUSA-N

Beschreibung

Allgemeine Beschreibung

A protective agent for SH groups.

Water-soluble protective reagent for sulfhydryl groups [absorbance (100 mM): ≤0.5 at 260 nm; ≤0.1 at 280 nm]. It is capable of maintaining monothiols completely in the reduced state and of reducing disulfides quantitatively. Since it readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Harmful (C)

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS05,GHS07
• Signalwort: Danger
• H-Sätze: H302,H315,H318
• P-Sätze: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable