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Association between cationic liposomes and low molecular weight hyaluronic acid.

Langmuir : the ACS journal of surfaces and colloids (2015-03-03)
Antonio A M Gasperini, Ximena E Puentes-Martinez, Tiago Albertini Balbino, Thais de Paula Rigoletto, Gabriela de Sá Cavalcanti Corrêa, Alexandre Cassago, Rodrigo Villares Portugal, Lucimara Gaziola de La Torre, Leide P Cavalcanti
ANOTACE

This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential.

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Sigma-Aldrich
L-α-Phosphatidylethanolamine, dioleoyl, ≥99% (GC), ≥98% (TLC), lyophilized powder
Sigma-Aldrich
1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine, ≥99.0% (10 mg phospholipid per ml CHCl3, TLC)
Sigma-Aldrich
1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine, ≥97.0% (TLC)