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  • Phytochemical analysis and in vitro biological activity of three Hypericum species from the Canary Islands (Hypericum reflexum, Hypericum canariense and Hypericum grandifolium).

Phytochemical analysis and in vitro biological activity of three Hypericum species from the Canary Islands (Hypericum reflexum, Hypericum canariense and Hypericum grandifolium).

Fitoterapia (2014-12-03)
Christian Zorzetto, Candelaria C Sánchez-Mateo, Rosa M Rabanal, Giulio Lupidi, Dezemona Petrelli, Luca A Vitali, Massimo Bramucci, Luana Quassinti, Giovanni Caprioli, Fabrizio Papa, Massimo Ricciutelli, Gianni Sagratini, Sauro Vittori, Filippo Maggi
ANOTACE

In the present work we carried out a phytochemical and biological investigation on three Hypericum species, i.e. Hypericum reflexum, Hypericum canariense and Hypericum grandifolium, from the Canary Islands where they are traditionally used as diuretic, wound healing, vermifuge, sedative and antidepressive agents. The polar extracts of the top flowering aerial parts, prepared by Soxhlet apparatus using a methanol-acetone (1:1) extracting mixture, were analyzed by HPLC-DAD and HPLC-MS for the content of eight biomarkers such as hypericin, hyperforin, chlorogenic acid, rutin, hyperoside, isoquercitrin, quercitrin and quercetin, whereas the hydrodistilled essential oils were analyzed by GC-FID and GC-MS. The three Hypericum species had different results in both polar and volatile constituents, H. reflexum being the only one endowed with a small amount of naphtodianthrones (hypericin and pseudohypericin), and containing high levels of chlorogenic acid, rutin and volatile mono- and sesquiterpenes. After chemical characterization, all products were in vitro biologically assayed for antiproliferative activity on human tumor cell lines by MTT assay, for antioxidant potential by DPPH, ABTS and FRAP assays, and for antimicrobial activity by the agar disc diffusion and microdilution methods. Results revealed interesting bioactivities and differences between polar extracts and essential oils, with the former being endowed with significant antioxidant activity and the latter with comparable inhibition effects on the tumor cells (A375, MDA-MB 231 and HCT 116) to that of cisplatin.

MATERIÁLY
Číslo produktu
Značka
Popis produktu

Hyperoside, primary reference standard
Chlorogenic acid, primary reference standard
USP
Palmitic acid, United States Pharmacopeia (USP) Reference Standard
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Eucalyptol, tested according to Ph. Eur.
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(−)-Terpinen-4-ol, ≥95.0% (sum of enantiomers, GC)
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Palmitic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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Palmitic acid, Pharmaceutical Secondary Standard; Certified Reference Material
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Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
Palmitic acid, European Pharmacopoeia (EP) Reference Standard
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Quercetin, ≥95% (HPLC), solid
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Eucalyptol, natural, ≥99%, FCC, FG
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Myrcene, technical grade
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Tridecane, ≥99%
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p-Cymene, ≥97%, FG
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Acetone, natural, ≥97%
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Undecane, ≥99%
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Heneicosane, 98%
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Tricosane, 99%
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Geraniol, analytical standard
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trans-Nerolidol, analytical standard
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Quercetin 3-β-D-glucoside, ≥90% (HPLC)
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Palmitic acid, analytical standard
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(+)-Terpinen-4-ol, analytical standard
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Acetone, analytical standard
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Methanol, analytical standard
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Myrcene, analytical standard
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Hexacosane, analytical standard
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Heptadecane, analytical standard
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Triacontane, analytical standard
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Tridecane, analytical standard