Přejít k obsahu
Merck
  • HAHAHA, HEHEHE, HIHIHI, or HKHKHK: influence of position and composition of histidine containing tags on biodistribution of [(99m)Tc(CO)3](+)-labeled affibody molecules.

HAHAHA, HEHEHE, HIHIHI, or HKHKHK: influence of position and composition of histidine containing tags on biodistribution of [(99m)Tc(CO)3](+)-labeled affibody molecules.

Journal of medicinal chemistry (2013-05-23)
Camilla Hofström, Mohamed Altai, Hadis Honarvar, Joanna Strand, Jennie Malmberg, Seyed Jalal Hosseinimehr, Anna Orlova, Torbjörn Gräslund, Vladimir Tolmachev
ANOTACE

Engineered affibody molecules can be used for high contrast in vivo molecular imaging. Extending a recombinantly produced HER2 binding affibody molecule with a hexa-histidine tag allows for convenient purification by immobilized metal-ion affinity chromatography and labeling with [(99m)Tc(CO)3](+) but increases radioactivity uptake in the liver. To investigate the impact of charge, lipophilicity, and position on biodistribution, 10 variants of a histidine-based tag was attached to a HER2 binding affibody molecule. The biochemical properties and the HER2 binding affinity appeared to be similar for all variants. In vivo, positive charge promoted liver uptake. For N-terminally placed tags, lipophilicity promoted liver uptake and decreased kidney uptake. Kidney uptake was higher for C-terminally placed tags compared to their N-terminal counterparts. The variant with the amino acid composition HEHEHE placed in the N-terminus gave the lowest nonspecific uptake.

MATERIÁLY
Číslo produktu
Značka
Popis produktu

Sigma-Aldrich
L-Histidine, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
L-Histidine, suitable for cell culture, meets EP, USP testing specifications, from non-animal source
Sigma-Aldrich
L-Histidine, ReagentPlus®, ≥99% (TLC)
Supelco
L-Histidine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
L-Histidine, Pharmaceutical Secondary Standard; Certified Reference Material
SAFC
L-Histidine