T2252

apo-Transferrin human

≥95% protein basis (biuret)

• Synonyma: Human transferrin, Siderophilin
• Číslo CAS: 11096-37-0
• EC Number: 234-318-8
• MDL number: MFCD00130536
• UNSPSC Code: 12352202
• NACRES: NA.61

Vlastnosti

• biological source: human
• Quality Level: 200
• assay: ≥95% protein basis (biuret); ≥98% (agarose gel electrophoresis)
• form: powder
• mol wt: 76-81 kDa
• technique(s): cell culture | mammalian: suitable
• solubility: H₂O: 50 mg/mL
• UniProt accession no.: P02787
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TF(7018)

Popis

General description

Transferrin is a bilobed glycoprotein containing an N-terminal lobe and a C-terminal lobe. Each of these domains is divided into two sub-domains and within the inter subdomain cleft is the metal-binding site. A single transferrin molecule can bind to two ferric ions. Apo-transferrin is an iron-free form.

Application

Apo-transferrin has been shown to strongly inhibit bacterial adhesion to biomaterials including polystyrene, polymethylmethacrylate, and silicone. Inhibition was seen at twenty micrograms/ml in culture medium.

apo-Transferrin human has been used in Dulbecco′s Modified Eagle Medium/Nutrient Mixture F-12 to culture:

• pancreatic ductal adenocarcinoma (PDAC) cell lines
• oligodendrocytes
• renal proximal tubule epithelial cells (RPTEC/TERT1) cells

Biochem/physiol Actions

Transferrin transports iron through receptor-mediated endocytosis. The iron transport mechanism occurs in the endothelial cells of the blood-brain barrier. Transferrin maintains iron homeostasis and is an iron-associated marker. Reduced levels of transferrin are observed in severe COVID-19 conditions. It may bind to other molecules apart from iron, hence it can be used in targeted drug delivery.

Other Notes

Non-heme iron-transport protein.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3