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Hlavní dokumenty

SRP3268

Sigma-Aldrich

PDGF-AA human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonyma:

GDGF, ODGF

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-3.0 ng/mL ED50

mol wt

28.5 kDa

packaging

pkg of 10 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P04085

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PDGFA(5154)

General description

Platelet derived growth factors (PDGFs) are disulfide-linked glycoprotein dimers consisting of two 12.0-13.5kDa polypeptide chains, designated as PDGF-A and PDGF-B chains. There are three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kDa disulfide-linked homodimer of two A chains (250 total amino acids).

Biochem/physiol Actions

The platelet derived growth factors (PDGFs) are stored in platelet a-granules and are released upon platelet activation. They are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. These proteins are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells.

Physical form

Lyophilized from 10 mM Acetic Acid.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
0212AFC03
0510AFC03

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