Přejít k obsahu
Merck
Všechny fotografie(2)

Hlavní dokumenty

SRP0396

Sigma-Aldrich

DNMT3A/DNMT3L active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

34 kDa (MLL1)
52 kDa (DNMT3L)

packaging

pkg of 10 μg

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

Související kategorie

General description

The DNMT3A (DNA (cytosine-5)-methyltransferase 3A) gene is mapped to human chromosome 2p23. The encoded protein has 2 isoforms, DNMT3A1 and DNMT3A2. DNMT3A1 is widely expressed and DNMT3A2 is mainly present in embryonic stem cells (ovaries and testes).
DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) belongs to the Dnmt3 family. It is unable to methylate DNA. The protein has a cysteine-rich region containing a novel-type zinc finger domain. The gene is mapped to human chromosome 21q22.3.

Application

DNMT3A/DNMT3L active human has been used in in vitro methylation assay and REMSA (RNA electrophoretic mobility shift assay) to study role of ecRNAs (extra coding RNAs) in DNA methylation.

Biochem/physiol Actions

DNMT3A (DNA (cytosine-5)-methyltransferase 3A) is a DNA methyltransferase. It is responsible for the methylation of the cytosine residue in 5′-C-phosphate-G-3′ (CpG) dinucleotides. DNMT3A plays a significant role in immunoregulation (adaptive as well as innate immune responses). It is also the most commonly mutated gene in hematologic malignancies. DNMT3A is a commonly mutated gene in acute myeloid leukemia and is responsible for an unfavorable prognosis.
DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) is mainly responsible for the stimulation of DNA methylation. It is a non-catalytic accessory factor. It helps the DNA methylation machinery to be placed on properly chromatinized DNA regions. It also helps the poorly methylated area, thereby providing uniform methylation patterns. Mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number

Nevidíte správnou verzi?

Potřebujete-li konkrétní verzi, můžete vyhledat daný certifikát podle čísla dávky nebo čísla šarže.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Association between single-nucleotide polymorphisms of DNMT3L and infertility with azoospermia in Chinese men.
Huang JX, et al.
Reproductive Biomedicine Online, 24, 66-66 (2012)
The CpG island encompassing the promoter and first exon of human DNMT3L gene is a PcG/TrX response element (PRE).
Basu A, et al.
PLoS ONE, 9, e93561-e93561 (2014)
DNMT3A Loss Drives Enhancer Hypomethylation in FLT3-ITD-Associated Leukemias.
Yang L, et al.
Cancer Cell, 29, 922-922 (2016)
Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family.
Aapola U, et al.
Genomics, 65, 293-293 (2000)
Gene-gene and gene-sex epistatic interactions of DNMT1, DNMT3A and DNMT3B in autoimmune thyroid disease.
Cai TT, et al.
Endocrine Journal, 63, 643-643 (2016)

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.