C3389

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type VI-S, lyophilized powder, 200-1,000 units/mg protein

• Synonyma: AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
• Číslo CAS: 9000-81-1
• UNSPSC Code: 12352204
• NACRES: NA.77
• EC Number: 232-559-3
• MDL number: MFCD00081268
• Číslo EC: 3.1.1.7 (BRENDA, IUBMB)
• Specific activity: 200-1,000 units/mg protein

Vlastnosti

• type: Type VI-S
• Quality Segment: 200
• form: lyophilized powder
• specific activity: 200-1,000 units/mg protein
• mol wt: 280 kDa
• composition: Protein, ≥45% biuret
• solubility: 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Popis

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E^1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H₂O to choline + acetic acid.

Physical form

Lyophilized powder containing Tris buffer salts

Preparation Note

This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Other Notes

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)