S9549

Superoxide dismutase–polyethylene glycol from bovine erythrocytes

lyophilized powder, ≥1,350 units/mg protein

• Synonyma: PEG-SOD
• MDL number: MFCD00678115
• UNSPSC Code: 12352204
• NACRES: NA.54

Vlastnosti

• form: lyophilized powder
• Quality Level: 200; 400
• specific activity: ≥1,350 units/mg protein
• mol wt: 5000 Da
• composition: Protein, ≥20% biuret
• extent of labeling: ≥10 mol PEG per mol protein
• storage temp.: −20°C

Popis

General description

Superoxide dismutase from bovine erythrocytes is a copper and zinc superoxide dismutase. Copper and zinc ions are coordinated by active site histidine residues. It has β-barrel structure.

Application

Superoxide dismutase-polyethylene glycol from bovine erythrocytes has been used:

• in the incubation of lung slices for measurement of dihydroethidium (DHE) fluorescence in cultured bovine pulmonary artery endothelial cells
• as supplement in T cell culture to investigate its effect on cytokine production
• in the preincubation of the carotid artery sections prior to DHE treatment

Biochem/physiol Actions

Superoxide dismutase-polyethylene glycol (SOD-PEG) is effective than SOD in scavenging free radicals in injuries. It reduces oxidative damage in ovarian follicles cell culture and during ovarian tissue transplantation. SOD-PEG is also effective in scavenging superoxide in mitochondrial abnormalities.

Packaging

Package size based on protein content

Other Notes

Superoxide dismutase from bovine erythrocytes coupled to methoxy-polyethylene glycol, M.W. 5,000, through secondary amine linkage.

Physical form

Lyophilized powder containing PEG plus 5% citrate buffer salts

Preparation Note

Enzyme derived

Bezpečnostní informace

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)