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S9396

Sigma-Aldrich

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Synonyma:

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

Číslo CAS:
9031-54-3
Číslo enzymu podle klasifikace EK:
3.1.4.12 (BRENDA, IUBMB)
MDL number:
MFCD00132362
UNSPSC Code:
12352204
NACRES:
NA.54

form

buffered aqueous glycerol solution

Quality Level

200

specific activity

≥100 units/mg protein (Lowry)

mol wt

34.2 kDa

foreign activity

Phospholipase C ≤1%

storage temp.

2-8°C

Gene Information

Bacillus cereus ATCC 10987 ... spH(2751530)

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General description

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
  • in lipoprotein modification for the generation of cholesterol crystals in vitro
  • to aggregate LDL (low-density lipoprotein)

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.

Unit Definition

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Extracellular lipids accumulate in human carotid arteries as distinct three-dimensional structures and have proinflammatory properties
Lehti S, et al.
The American Journal of Pathology, 188(2), 525-538 (2018)
The Bacillus cereus group
Molecular Medical Microbiology, 1041-1078 (2015)
Analysis of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines
Otsuki N, et al.
Journal of virology (2017)
G Pabst et al.
European biophysics journal : EBJ, 41(10), 915-929 (2012-06-05)
Biological membranes can be targets for compounds that either disrupt their barrier function or affect protein function via membrane-mediated processes. Biophysical studies on membrane-mimetic systems composed of membrane lipids have contributed substantially to our knowledge on the pertaining membrane physics
Raymond Tan et al.
Experimental and molecular pathology, 94(1), 51-57 (2012-06-23)
The HIV-1 accessory protein Nef is considered to play an important role in the development of a podocyte phenotype in HIV-1 associated nephropathy. We hypothesized that Nef may be altering the podocyte phenotype both structurally and functionally. To elucidate the
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