Přejít k obsahu
Merck
Všechny fotografie(2)

Key Documents

R2520

Sigma-Aldrich

Ribonuclease inhibitor human

Inhibits ribonucleases during transcription and translation experiments

Synonyma:

RNase Inhibitor, Ribonuclease Inhibitor

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

MDL number:
UNSPSC Code:
12352200
NACRES:
NA.52

biological source

human placenta

grade

for molecular biology

form

buffered aqueous solution

mol wt

~50 kDa

concentration

30,000-50,000 units/mL

shipped in

dry ice

storage temp.

−20°C

Hledáte podobné produkty? Navštivte Průvodce porovnáváním produktů

General description

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is human placenta. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes.

Application

Suitable for use in
  • in vitro inhibition of ribonucleases
  • cDNA synthesis
  • RT-PCR
  • in vitro transcription and translation
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Biochem/physiol Actions

Inhibits RNase by forming a tight, non-covalent 1:1 complex. Also inhibits angiogenin, a neovascularization promoter which is homologous with RNase A.

Components

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Other Notes

This inhibitor of RNase activity is isolated from human placenta and supplied as a buffered aqueous glycerol solution.
Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT

Unit Definition

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Physical form

Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT

replaced by

Č. produktu
Popis
Stanovení ceny

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Mariona Estapé Senti et al.
Journal of controlled release : official journal of the Controlled Release Society, 341, 475-486 (2021-12-11)
PEGylation of lipid-based nanoparticles and other nanocarriers is widely used to increase their stability and plasma half-life. However, either pre-existing or de novo formed anti-PEG antibodies can induce hypersensitivity reactions and accelerated blood clearance through binding to the nanoparticle surfaces
Sabahattin Kaymakoglu et al.
Antimicrobial agents and chemotherapy, 49(3), 1135-1138 (2005-02-25)
The success of alpha interferon (IFN-alpha) monotherapy for the treatment of chronic hepatitis D is very limited. In this study, the efficacy of IFN-alpha and ribavirin combination therapy for chronic hepatitis D was investigated. Nineteen patients (15 males; mean age
Ribonuclease inhibitor from human placenta: interaction with derivatives of ribonuclease A.
P Blackburn et al.
The Journal of biological chemistry, 254(24), 12488-12493 (1979-12-25)
Michal Mikula et al.
The Journal of biological chemistry, 286(11), 9763-9775 (2011-01-15)
Components of the ERK cascade are recruited to genes, but it remains unknown how they are regulated at these sites. The RNA-binding protein heterogeneous nuclear ribonucleoprotein (hnRNP) K interacts with kinases and is found along genes including the mitogen-inducible early
Malia Su-Qin Murphy et al.
American journal of obstetrics and gynecology, 213(3), 367-367 (2015-05-20)
Preeclampsia (PE) is a hypertensive disorder of pregnancy characterized by widespread maternal endothelial dysfunction. Although clinical signs subside following delivery, long-term risks associated with PE include hypertension, stroke, and cardiovascular disease. MicroRNAs (miRNAs) are emerging as critical regulators of biological

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.