MSST0022

SILu^™Lite CRP C-reactive Protein human

recombinant, expressed in HEK 293 cells, MS Protein Standard

• Synonyma: PTX1, pentaxin, pentraxin
• UNSPSC Code: 23201100
• NACRES: NA.12

Vlastnosti

• biological source: human
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• assay: ≥98% (SDS-PAGE)
• form: liquid
• technique(s): mass spectrometry (MS): suitable
• suitability: suitable for mass spectrometry (internal calibrator)
• UniProt accession no.: P02741
• storage temp.: −20°C
• Gene Information: human ... PTX3(5806)

Popis

General description

SILu^™Lite CRP is a recombinant human protein expressed in human 293 cells. It is a protein consisting of 226 amino acids (including a C-terminal polyhistidine and FLAG^® tags), with a calculated molecular weight of ~25 kDa. SILu^™Lite CRP is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.


Suggested Quantitative Analysis Parameters
(MRM settings provided for three suggested peptides)

Biochem/physiol Actions

CRP is a hepatically derived pentraxin. It exists mainly as a pentamer in a cyclic structure with 5 identical 23-kD subunits in the blood. In addition to its role as an "acute phase" protein or first-line defense molecule against pathogenic organisms, baseline levels of CRP correlate positively to adverse cardiovascular events. CRP is therefore being considered a “risk factor” for cardiovascular disease. In humans, treatment with statin therapy reduces levels of both LDL-C and CRP, and concurrently there is a reduction in the number of cardiovascular events. A recent study suggests that patients with elevated basal levels of CRP are at an increased risk of diabetes, in addition to cardiovascular disease. A study of over 700 nurses showed that those in the highest quartile of trans fat consumption had blood levels of CRP that were 73% higher than those in the lowest quartile.

Sequence

QTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWPDYKDDDDKGHHHHHHHHGGQ

Physical form

Supplied as a solution of phosphate buffered saline with 25% glycerol.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

SILu is a trademark of Sigma-Aldrich Co. LLC

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable