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MOD50

Sigma-Aldrich

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

Synonyma:

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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About This Item

EC Number:
231-548-0
UNSPSC Code:
41116158
NACRES:
NA.54

Quality Level

200

usage

sufficient for 50 reactions

storage temp.

20-25°C

General description

The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

Application

Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.

Features and Benefits

  • Only 50 picograms of DNA or 20 cells are required
  • Procedure takes less than 2 hours
  • Greater than 99% conversion rate
  • Extremely low degradation
  • Option of convenient one-step protocol
  • Consistent and reproducible Bisulfite Modification
  • Can be used with genomic, endonuclease digested, and FFPE DNA

Storage and Stability

All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list
  • T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, naturalBezpečností list

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

supp_hazards

EUH031

Storage Class

8B - Non-combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
0000333642
0000333642
SLCQ0447
SLCD4557
SLCD4559

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Potřebujete-li konkrétní verzi, můžete vyhledat daný certifikát podle čísla dávky nebo čísla šarže.

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Sortimentní položky

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

Protokoly

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Související obsah

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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