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M4892

SAFC

McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Farmaceutická výroba

Synonyma:

5A basal medium

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About This Item

MDL number:
MFCD00217560
UNSPSC Code:
12352207
NACRES:
NA.71

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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General description

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used for culturing:
  • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
  • human colorectal HCT116 cells to study miR-224 over-expression in cancer
  • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Quantity

Formulated to contain 11.9 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

also commonly purchased with this product

Č. produktu
Popis
Stanovení ceny

supplement

Č. produktu
Popis
Stanovení ceny

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Sortimentní položky

McCoy's 5A Modified Media Formulation

McCoy's 5A Modified Media Formulation

Protokoly

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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