L3382
Lectin from Helix pomatia
lyophilized powder, salt, free
Synonyma:
Helix pomatia agglutinin, HPA
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Doporučené produkty
form
lyophilized powder
potency
<4 μg per mL agglutination activity
impurities
salt, free
storage temp.
−20°C
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General description
Lectin from Helix pomatia is a hexameric glycoprotein with a molecular mass of 79 kDa. It issecreted by the albumen gland of the roman snail. Helix pomatia agglutinin (HPA) is specific for N-acetylgalactosamine (GalNAc).
Application
Lectin from Helix pomatia has been used to conjugate with biotin and acridinium and used in lectin-agglutination tests.
Biochem/physiol Actions
Lectin from Helix pomatia protects fertilized eggs by coating them with perivitelline fluid. They exhibit antibacterial activities and immunity in snail. Helix pomatia agglutinin (HPA) possess the potential to agglutinate pathogens. HPA has also emerged as a marker of altered Oglycosylation in cancer.
HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Preparation Note
Affinity purified by a modification of the method of Hammarstrom and Kabat.
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Osvědčení o analýze (COA)
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Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Hammarstrom, S., and Kabat, E.A.
Biochemistry, 95, 311-311 (1978)
Characterization of bovine and human group B streptococci isolated in Turkey
Ekin IH and Gurturk K
Journal of Medical Microbiology, 55(5), 517-521 (2006)
Katie Barr et al.
Transfusion, 55(1), 129-136 (2014-07-22)
The FORS blood group system (originally recognized as the Apae phenotype) was discovered by sporadic activity against polyclonal anti-A reagents and activity against the lectin Helix pomatia. The extent of monoclonal anti-A reagent activity against the FORS1 antigen is serologically
Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients
Reily C, et al.
Biotechniques, 65(2), 71-77 (2018)
Binding of aberrant glycoproteins recognizable by Helix pomatia agglutinin in adrenal cancers
Parameswaran R, et al.
BJS open, 2(5), 353-359 (2018)
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