H9661

Hepatocyte Growth Factor human

≥95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Synonyma: HGF, Hepatopoietin A, Scatter Factor
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00217422
• Biological source: human
• Recombinant: expressed in NSO cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: 70-80 kDa by SDS-PAGE (non-reducing)
• Impurities: ≤4 ng/mL (ED50)

Vlastnosti

• Název produktu: Hepatocyte Growth Factor human, HGF, recombinant, expressed in NSO cells, suitable for cell culture
• biological source: human
• Quality Segment: 200
• recombinant: expressed in NSO cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: 20-40 ng/mL
• quality: endotoxin tested
• mol wt: 70-80 kDa by SDS-PAGE (non-reducing)
• packaging: pkg of 5 and 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤4 ng/mL (ED50)
• UniProt accession no.: P14210
• storage temp.: −20°C
• Gene Information: human ... HGF(3082)

Popis

General description

Recombinant human Hepatocyte Growth Factor (HGF) is expressed in NSO cells and supplied as a lyophilized protein suitable for mammalian cell culture research. HGF is a cytokine that signals through the c‑Met receptor and is widely studied in in vitro cell models.

Application

Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells. It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.

Biochem/physiol Actions

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor that exhibits mitogenic and motogenic activity in vitro and mediates cellular responses via c‑Met receptor signaling pathways.[1] It′s major function is to induce in vitro hepatocytes proliferation. HGF is also essential for cell migration and angiogenesis. Itis associated with embryonic development and tissue homeostasis. It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.

Analysis Note

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• Co se děje?: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)