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GL0010

Sigma-Aldrich

Golgi Isolation Kit

sufficient for 50 g (tissue)

Synonyma:

Golgi Kit, Isolation Kit for Golgi

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

usage

sufficient for 50 g (tissue)

Quality Level

technique(s)

fractionation: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).

Application

Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.

Analysis Note

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLBezpečností list

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Sortimentní položky

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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