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GE17-5442-03

Ficoll®-Paque Premium

Cytiva 17-5442-03, pack of 6 × 500 mL

Synonyma:

Ficoll density gradient medium, cell culture media

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.25

form

liquid

packaging

pack of 6 × 500 mL

manufacturer/tradename

Cytiva 17-5442-03

color

Colorless to slightly yellow

pH range

5.5-7.5

General description

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

Application

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

Features and Benefits

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class

12 - Non Combustible Liquids

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Osvědčení o analýze (COA)

Lot/Batch Number

Je nám líto, ale pro tento produkt momentálně nemáme COA k dispozici online.

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Sortimentní položky

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

How to Fractionate and Analyze Gradients of Percoll

Density Marker Beads facilitate Percoll gradient monitoring in biological separations.

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Protokoly

Recommended Standard Method for Isolating Mononuclear Cells

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Související obsah

Applications of Percoll In Blood Cells

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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